Department of Orthopaedics & Traumatology, Provincial Hospital Affiliated with Shandong University, Ji'nan 250021, Shandong, People's Republic of China.
J Orthop Res. 2010 Feb;28(2):252-7. doi: 10.1002/jor.20969.
In bone tissue engineering, the cell distribution mode in the scaffold may affect in vivo osteogenesis. Therefore, we fabricated a novel biomimetic construct based on a combination of rabbit adipose-derived stem cells (rASCs) encapsulated in collagen I gel with a PLGA-beta-TCP scaffold (rASCs-COL/PLGA-beta-TCP, group A), the combination of rASCs and PLGA-beta-TCP (rASCs/PLGA-beta-TCP, group B), the combination of collagen I gel and PLGA-beta-TCP (COL/PLGA-beta-TCP, group C), and PLGA-beta-TCP scaffold (group D). The composites were implanted into a 15-mm length critical-sized segmental radial defect. The results were assessed by histology, radiographs, bone mineral density (BMD), and mechanical testing. After 24 weeks, the medullary cavity recanalized, bone was rebuilt, and molding finished, the bone contour remodeled smoothly and the scaffold degraded completely in group A. The BMDs and mechanical properties were similar to normal. However, the bone defect remained unrepaired in groups B, C, and D. Moreover, the scaffold degradation rate in group A was significantly higher than the other groups. Thus, enhanced in vivo osteogenesis of rASCs wrapped in collagen I gel combined with PLGA-beta-TCP was achieved, and the bone defect was repaired. We hope this study provides new insights into ASCs-based bone tissue engineering.
在骨组织工程中，支架中的细胞分布模式可能会影响体内成骨。因此，我们构建了一种新型仿生构建体，它基于胶原蛋白 I 凝胶包裹的兔脂肪来源干细胞 (rASCs) 与 PLGA-β-TCP 支架的结合（rASCs-COL/PLGA-β-TCP，A 组）、rASCs 和 PLGA-β-TCP 的结合（rASCs/PLGA-β-TCP，B 组）、胶原蛋白 I 凝胶和 PLGA-β-TCP 的结合（COL/PLGA-β-TCP，C 组）和 PLGA-β-TCP 支架（D 组）。将这些复合材料植入 15mm 长的临界尺寸节段性桡骨缺损中。通过组织学、射线照相、骨矿物质密度 (BMD) 和机械测试评估结果。24 周后，骨髓腔再通，重建了骨骼，完成了塑形，A 组的骨轮廓平滑重塑，支架完全降解。BMD 和机械性能与正常相似。然而，B、C 和 D 组的骨缺损仍未修复。此外，A 组的支架降解率明显高于其他组。因此，包裹在胶原蛋白 I 凝胶中的 rASCs 与 PLGA-β-TCP 结合可增强体内成骨作用，修复骨缺损。我们希望这项研究为基于 ASC 的骨组织工程提供新的见解。
