Plos K, Carter T, Hull S, Hull R, Svanborg Edén C
Department of Clinical Immunology, University of Göteborg, Sweden.
J Infect Dis. 1990 Mar;161(3):518-24. doi: 10.1093/infdis/161.3.518.
The pap operon encodes the gal alpha 1-4gal beta specific adhesins of Escherichia coli. The presence and organization of pap homologous DNA was determined using two probes specific for pap in 217 uropathogenic E. coli samples by dot blot and Southern blot analysis. The frequency of pap homologous DNA was 76% in pyelonephritis, 69% in cystitis, and 52% in an asymptomatic bacteriuria group. Further, the gal alpha 1-4gal beta binding phenotype among the pap-positive strains was expressed more often in acute pyelonephritis (91%) than the cystitis (60%) or asymptomatic bacteriuria (52%) strains. This was explained in part by difference in organization of pap homologous DNA between the genotypically positive pyelonephritis and asymptomatic bacteriuria strains. The pyelonephritis isolates contained three copies of pap significantly more often than the asymptomatic bacteriuria strains, and the pyelonephritogenic O-antigen types had a general increase in pap copy number. The difference in expression of gal alpha 1-4gal beta adhesins between pyelonephritis and asymptomatic bacteriuria isolates was thus not only a function of the frequency of pap homologous DNA but also of phenotypic expression among genotypically pap-positive strains.
pap操纵子编码大肠杆菌的α1-4半乳糖基-β-半乳糖特异性粘附素。通过斑点印迹和Southern印迹分析，使用两种对pap特异的探针，在217份致病性大肠杆菌样本中确定了pap同源DNA的存在和组织情况。在肾盂肾炎中，pap同源DNA的频率为76%，膀胱炎中为69%，无症状菌尿组中为52%。此外，pap阳性菌株中的α1-4半乳糖基-β-半乳糖结合表型在急性肾盂肾炎（91%）中比膀胱炎（60%）或无症状菌尿（52%）菌株中更常表达。这部分是由于基因型阳性的肾盂肾炎和无症状菌尿菌株之间pap同源DNA组织的差异。肾盂肾炎分离株比无症状菌尿菌株更频繁地含有三个拷贝的pap，并且致肾盂肾炎的O抗原类型的pap拷贝数普遍增加。因此，肾盂肾炎和无症状菌尿分离株之间α1-4半乳糖基-β-半乳糖粘附素表达的差异不仅是pap同源DNA频率的函数，也是基因型pap阳性菌株中表型表达的函数。
