Plos K, Lomberg H, Hull S, Johansson I, Svanborg C
Department of Clinical Immunology, University of Göteborg, Sweden.
Pediatr Infect Dis J. 1991 Jan;10(1):15-9.
The frequency of Escherichia coli with Gal alpha 1-4Gal beta-specific adhesins is reduced among children who develop renal scars. The adhesion-negative phenotype may be due to the absence of the pap DNA sequences which encode these adhesins or to a phase variation event induced by in vitro culture. In the present study the frequency of pap and pil homologous DNA was determined by dot blot analysis with probes specific for the respective sequence using E. coli strains from children with recurrent pyelonephritis with and without renal scarring. The frequency of pap was 79% in the strains isolated from the nonscarring group compared with 39% in the strains from the scarring group (P less than 0.001). The Gal alpha 1-4Gal beta phenotype was expressed by 89% of the pap-positive strains from the nonscarring group compared with 71% in the scarring group (P less than 0.05). In addition 13 of 77 of the pap-positive E. coli strains agglutinated sheep erythrocytes but not the Gal alpha 1-4Gal beta latex beads; a reaction attributed to reactivity with the Forssman glycolipid. DNA sequences homologous with pil were found in 95% of all strains and there was no significant difference between the nonscarring and the scarring groups. The low frequency of Gal alpha 1-4Gal beta specific strains in the scarring group was therefore due to the absence of pap-homologous DNA sequences and to a reduced rate of phenotypic expression among pap-positive scarring strains. There was no support for a relationship between type 1 fimbriae and renal scarring.
在出现肾瘢痕的儿童中，具有α1-4半乳糖基-β-特异性黏附素的大肠杆菌频率降低。黏附阴性表型可能是由于编码这些黏附素的pap DNA序列缺失，或者是由体外培养诱导的相变事件所致。在本研究中，使用来自有或无肾瘢痕的复发性肾盂肾炎儿童的大肠杆菌菌株，通过斑点印迹分析，用针对各自序列的特异性探针来确定pap和菌毛同源DNA的频率。在从无瘢痕组分离的菌株中，pap的频率为79%，而在有瘢痕组的菌株中为39%（P<0.001）。无瘢痕组中89%的pap阳性菌株表达α1-4半乳糖基-β-表型，而有瘢痕组中为71%（P<0.05）。此外，77株pap阳性大肠杆菌菌株中有13株凝集绵羊红细胞，但不凝集α1-4半乳糖基-β-乳胶珠；这种反应归因于与福斯曼糖脂的反应性。在所有菌株中有95%发现了与菌毛同源的DNA序列，无瘢痕组和有瘢痕组之间没有显著差异。因此，有瘢痕组中α1-4半乳糖基-β-特异性菌株的低频率是由于缺乏pap同源DNA序列以及pap阳性有瘢痕菌株中表型表达率降低。没有证据支持1型菌毛与肾瘢痕之间的关系。
