Archambaud M, Courcoux P, Labigne-Roussel A
Laboratoire de Bactériologie-Virologie, CHU Rangueil, Toulouse, France.
Ann Inst Pasteur Microbiol. 1988 Sep-Oct;139(5):575-88. doi: 10.1016/0769-2609(88)90156-1.
The genetic determinants responsible for the adherence of Escherichia coli to uroepithelial cells have been identified in recent years by genetic and molecular methods. Specific DNA probes for each of the three operons which have been cloned so far (pap, afa, sfa/foc operons) have been used in colony hybridization experiments to detect the presence of each of these operons in the chromosomal DNA of 443 strains of E. coli; 186 strains were from patients with urinary tract infections (pyelonephritis, 106 strains; cystitis, 59; asymptomatic bacteriuria, 21) and 257 were strains from the stools of healthy subjects (61) or from patients with various enteral infections (196). E. coli strains harbouring the pap operon were found more frequently in the urine of patients with pyelonephritis (p less than 0.001) and cystitis (p less than 0.01) than in control stools. The presence of two operons (pap + afa) or (pap + sfa/foc) was only observed in uropathogenic strains (p less than 0.02). Pap and sfa/foc operons were never found in strains causing enteral infection; however, the afa operon was found in 7.6% of the enteropathogenic E. coli.
近年来，通过遗传学和分子学方法已鉴定出负责大肠杆菌黏附于尿道上皮细胞的遗传决定因素。针对目前已克隆的三个操纵子（pap、afa、sfa/foc操纵子）中的每一个，其特异性DNA探针已用于菌落杂交实验，以检测443株大肠杆菌染色体DNA中这些操纵子的存在情况；186株来自尿路感染患者（肾盂肾炎患者106株；膀胱炎患者59株；无症状菌尿患者21株），257株来自健康受试者的粪便（61株）或患有各种肠道感染的患者（196株）。与对照粪便相比，携带pap操纵子的大肠杆菌菌株在肾盂肾炎患者（p<0.001）和膀胱炎患者（p<0.01）尿液中更为常见。仅在尿路致病性菌株中观察到两个操纵子（pap + afa）或（pap + sfa/foc）的存在（p<0.02）。在引起肠道感染的菌株中从未发现过Pap和sfa/foc操纵子；然而，在7.6%的肠道致病性大肠杆菌中发现了afa操纵子。
