Hennessy Daniel N, Narayanan Beena, Rosenberg John M
Department of Biological Sciences, 312 Clapp Hall, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Acta Crystallogr D Biol Crystallogr. 2009 Sep;65(Pt 9):1001-3. doi: 10.1107/S0907444909020320. Epub 2009 Aug 14.
Crystallization trials can be designed as a systematic gradient of the concentration of key reagents and/or pH centered on the original conditions. While the concept of the grid screen is simple, its implementation is tedious and difficult by hand. A procedure has been developed for preparing crystallization grid screens that is both efficient and achieves high accuracy because it relies on a limited number of solutions that are carefully prepared by hand. The ;four-corners' approach to designing grid screens uses the minimum and maximum concentrations of the components being varied in the grid screen as the sole stock solutions. For an N-dimensional grid only 2(N) corner solutions require detailed preparation, making the screens efficient. Furthermore, by keeping the concentrations as tight as possible to the grid, the potential impact of pipette errors is minimized, creating a highly precise screen.
结晶试验可以设计成以原始条件为中心的关键试剂浓度和/或pH值的系统梯度。虽然网格筛选的概念很简单，但手工实施起来既繁琐又困难。已经开发出一种制备结晶网格筛选的程序，该程序既高效又能实现高精度，因为它依赖于少量手工精心制备的溶液。设计网格筛选的“四角”方法使用网格筛选中变化的组分的最小和最大浓度作为唯一的储备溶液。对于N维网格，仅需要详细制备2(N)种角溶液，从而使筛选高效。此外，通过使浓度尽可能接近网格，移液误差的潜在影响最小化，从而创建高度精确的筛选。
