含有多聚TG的大限制性片段在多种脊椎动物中具有高度多态性。
Large restriction fragments containing poly-TG are highly polymorphic in a variety of vertebrates.
作者信息
Kashi Y, Tikochinsky Y, Genislav E, Iraqi F, Nave A, Beckmann J S, Gruenbaum Y, Soller M
机构信息
Department of Genetics, Hebrew University, Jerusalem, Israel.
出版信息
Nucleic Acids Res. 1990 Mar 11;18(5):1129-32. doi: 10.1093/nar/18.5.1129.
Southern blots of genomic DNA from a variety of species digested by restriction endonucleases having a four-bp specificity, were probed with a bovine genomic clone consisting of seven tandem poly-TG stretches separated by a 29bp linker sequence. Highly variable DNA 'fingerprint' patterns were obtained in chicken, sheep, and horse, moderately variable DNA 'fingerprints' in mouse and man, and a monomorphic pattern in Drosophila. In chicken, horse and man a (TG)10 synthetic oligonucleotide probe gave results identical to those given by the bovine probe. Furthermore, in chicken the DNA fingerprint variation showed typical Mendelian inheritance and differed from the fingerprints obtained with Jeffreys 33.6 and M13 minisatellite probes. Thus, for a variety of vertebrate species, poly-TG-containing probes can uncover useful genetic variation.
用具有四碱基特异性的限制性内切酶消化多种物种的基因组DNA，进行Southern杂交，用一个由七个串联的多聚TG序列组成、中间间隔29bp连接序列的牛基因组克隆作为探针。在鸡、绵羊和马中获得了高度可变的DNA“指纹”图谱，在小鼠和人中获得了中等可变的DNA“指纹”，而在果蝇中则是单态图谱。在鸡、马和人中，一个（TG）10合成寡核苷酸探针给出的结果与牛探针相同。此外，在鸡中，DNA指纹变异显示出典型的孟德尔遗传，并且与用杰弗里斯33.6和M13微卫星探针获得的指纹不同。因此，对于多种脊椎动物物种，含多聚TG的探针可以揭示有用的遗传变异。
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