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在哺乳动物核糖体DNA和两个X连锁基因中与聚（dG-dT）·（dC-dA）杂交的序列的位置和背景

Locations and contexts of sequences that hybridize to poly(dG-dT).(dC-dA) in mammalian ribosomal DNAs and two X-linked genes.

作者信息

Braaten D C, Thomas J R, Little R D, Dickson K R, Goldberg I, Schlessinger D, Ciccodicola A, D'Urso M

机构信息

Department of Microbiology and Immunology, Washington University School of Medicine, St Louis, MO 63110.

出版信息

Nucleic Acids Res. 1988 Feb 11;16(3):865-81. doi: 10.1093/nar/16.3.865.

DOI:10.1093/nar/16.3.865
PMID:3267216
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334724/
Abstract

Sequences located several kilobases both 5' and 3' of the stably transcribed portion of several genes hybridize to radio-labeled pure fragments of the alternating sequence poly (dG-dT) (dC-dA) ["poly(GT)"]. The genes include the ribosomal DNA of mouse, rat, and human, and also human glucose-6-phosphate dehydrogenase (G6PD) and mouse hypoxanthine-guanine phosphoribosyl transferase (HPRT). HPRT has additional hybridizing sequences in introns. Fragments that include the hybridizing sequences and up to 300 bp of adjoining DNA show perfect runs of poly(GT) (greater than 30bp) in all but the human 5' region of rDNA, which shows a somewhat different alternating purine:pyrimidine sequence, poly(GTAT) (36bp). Within 150 bp of these sequences in various instances are found a number of other sequences reported to affect DNA conformation in model systems. Most marked is an enhancement of sequences matching at least 67% to the consensus binding sequence for topoisomerase II. Two to ten-fold less of such sequences were found in other sequenced portions of the nontranscribed spacer or in the transcribed portion of rDNA. The conservation of the locations of tracts of alternating purine:pyrimidine between evolutionarily diverse species is consistent with a possible functional role for these sequences.

摘要

在几个基因稳定转录部分的5'端和3'端数千碱基处的序列，与交替序列聚（dG-dT）（dC-dA）["聚（GT）"]的放射性标记纯片段杂交。这些基因包括小鼠、大鼠和人类的核糖体DNA，以及人类葡萄糖-6-磷酸脱氢酶（G6PD）和小鼠次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HPRT）。HPRT在内含子中有额外的杂交序列。包含杂交序列和多达300 bp相邻DNA的片段，除了rDNA的人类5'区域显示出有点不同的交替嘌呤：嘧啶序列聚（GTAT）（36 bp）外，在所有其他区域都显示出完美的聚（GT）连续序列（大于30 bp）。在各种情况下，在这些序列的150 bp范围内发现了许多其他据报道在模型系统中影响DNA构象的序列。最显著的是与拓扑异构酶II的共有结合序列至少67%匹配的序列增强。在非转录间隔区的其他测序部分或rDNA的转录部分中发现的此类序列减少了两到十倍。进化上不同物种之间交替嘌呤：嘧啶片段位置的保守性与这些序列可能的功能作用一致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c483/334724/29556f16ff74/nar00145-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c483/334724/4568a0a81639/nar00145-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c483/334724/29556f16ff74/nar00145-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c483/334724/4568a0a81639/nar00145-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c483/334724/29556f16ff74/nar00145-0098-a.jpg

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