Department of Neuroscience, Unit of Functional Pharmacology, Uppsala University, BMC, Uppsala, P. O. Box 593, SE 75124, Sweden.
J Mol Neurosci. 2010 Jun;41(2):230-42. doi: 10.1007/s12031-009-9222-7. Epub 2009 Aug 21.
About one third of all known human proteins are membrane proteins, which constitute several large families. The solute carriers with over 300 known members are probably the second largest family with additional members frequently being identified. We recently found a new putative solute carrier, C6ORF192, belonging to the major facilitator superfamily type of proteins. The gene is evolutionary highly conserved with a single copy present in each of the genomes from mouse, rat, chicken, zebrafish, tetraodon, Caenorhabditis elegans, and Drosophila melanogaster. C6ORF192 forms a novel evolutionary branch of solute carriers and is most closely related to the solute carrier families 16, 17, and 18, all members of the major facilitator superfamily. Ten of the 25 members of these families show amino acid identity with C6ORF192 ranging from 21% to 27%. C6ORF192 differs however, structurally from these families and does not share key motifs in the transmembrane domains. Expression profiling by quantitative real-time polymerase chain reaction and in situ hybridization showed that C6ORF192 transcript can be detected in several tissues, both in the central nervous system and the periphery.
约三分之一的已知人类蛋白为膜蛋白，这些蛋白构成了几个大的家族。溶质载体蛋白家族有超过 300 个已知成员，可能是第二大的家族，并且还在不断发现新成员。我们最近发现了一个新的假定溶质载体蛋白 C6ORF192，它属于主要协助因子超家族蛋白。该基因在进化上高度保守，在从鼠、大鼠、鸡、斑马鱼、甲鱼、秀丽隐杆线虫和黑腹果蝇的每个基因组中都存在一个单一的拷贝。C6ORF192 形成了溶质载体蛋白的一个新的进化分支，与溶质载体家族 16、17 和 18 最为密切相关，它们都是主要协助因子超家族的成员。这三个家族的 25 个成员中有 10 个与 C6ORF192 具有氨基酸同一性，范围从 21%到 27%。然而，C6ORF192 在结构上与这些家族不同，并且在跨膜结构域中不共享关键基序。通过实时定量聚合酶链反应和原位杂交进行的表达谱分析表明，C6ORF192 转录本可以在几种组织中检测到，包括中枢神经系统和外周组织。
