da Silva B B, Lopes-Costa P V, dos Santos A R, de Sousa-Júnior E C, Alencar A P, Pires C G, Rosal M A
Department of Gynecology, Mastology Division, Federal University of Piauí, Teresina, Piauí, Brazil.
Eur J Gynaecol Oncol. 2009;30(3):285-8.
To evaluate the microvessel density by comparing the performance of anti-factor VIII-related antigen, anti-CD31 and anti-CD34 monoclonal antibodies in breast cancer.
Twenty-three postmenopausal women diagnosed with Stage II breast cancer submitted to definitive surgical treatment were evaluated. The monoclonal antibodies used were anti-factor VIII, anti-CD31 and anti-CD34. Microvessels were counted in the areas of highest microvessel density in ten random fields (200 x). The data were analyzed using the Kruskal-Wallis nonparametric test (p < 0.05).
Mean microvessel densities with anti-factor VIII, anti-CD31 and anti-CD34 were 4.16 +/- 0.38, 4.09 +/- 0.23 and 6.59 +/- 0.42, respectively. Microvessel density as assessed by anti-CD34 was significantly greater than that detected by anti-CD31 or anti-factor VIII (p < 0.0001). There was no statistically significant difference between anti-CD31 and anti-factor VIII (p = 0.4889).
The density of stained microvessels was greater and staining was more intense with anti-CD34 compared to anti-CD31 and anti-factor VIII-related antigen.
通过比较抗 VIII 因子相关抗原、抗 CD31 和抗 CD34 单克隆抗体在乳腺癌中的表现来评估微血管密度。
对 23 例确诊为 II 期乳腺癌并接受确定性手术治疗的绝经后女性进行评估。使用的单克隆抗体为抗 VIII 因子、抗 CD31 和抗 CD34。在十个随机视野（200 倍）中微血管密度最高的区域计数微血管。使用 Kruskal-Wallis 非参数检验分析数据（p < 0.05）。
抗 VIII 因子、抗 CD31 和抗 CD34 检测的平均微血管密度分别为 4.16±0.38、4.09±0.23 和 6.59±0.42。抗 CD34 评估的微血管密度显著高于抗 CD31 或抗 VIII 因子检测到的密度（p < 0.0001）。抗 CD31 和抗 VIII 因子之间无统计学显著差异（p = 0.4889）。
与抗 CD31 和抗 VIII 因子相关抗原相比，抗 CD34 染色的微血管密度更高且染色更强。
