Orre M, Lotfi-Miri M, Mamers P, Rogers P A
Monash University Department of Obstetrics and Gynaecology Monash Medical Centre, Clayton, Victoria, Australia.
Br J Cancer. 1998 Jun;77(12):2204-9. doi: 10.1038/bjc.1998.367.
Microvessel density of benign, borderline and malignant ovarian tumours was studied immunohistochemically using antibodies to the endothelial cell markers CD31, CD34 and factor VIII-related antigen. Microvessel density was compared in tumours of different histological subtype, stage and patient outcome. CD31-immunostained sections were examined and regions of high and average microvessel density were selected. Identical regions were located on CD34- and factor VIII-related antigen-immunostained serial sections and microvessel counts obtained and converted to vessels mm(-2). CD31 and CD34 immunostaining revealed increased microvessel density in both the high and average vessel density regions of mucinous (222.4 +/- 24.8; 79.9 +/- 8.5) compared with serous (105.4 +/- 20.7; 33.3 +/- 6.8) and benign (84.4 +/- 19.4; 20.4 +/- 4.4) tumours (P < 0.001). CD31 and CD34 immunostaining also revealed increased microvessel density in early-stage mucinous tumours (234.6 +/- 28.2; 87.8 +/- 9.2) compared with that observed in both early- (72.8 +/- 15; 12.9 +/- 2.4) and late- (115.6 +/- 26.5; 29.8 +/- 8.5) stage serous tumours (P < 0.001). No differences in microvessel density in samples from patients with differing outcomes were observed (P > 0.05). Reduced factor VIII-related antigen compared with CD31 and CD34 immunostaining was observed in both borderline and malignant mucinous and serous tumours (P < 0.02) but not in benign tumours (P > 0.05). Our results contradict the putative association between increased microvessel density and poor prognosis and suggest that the level and control of angiogenesis may differ between ovarian tumour types.
采用抗内皮细胞标志物CD31、CD34和VIII因子相关抗原的抗体，通过免疫组织化学方法研究了良性、交界性和恶性卵巢肿瘤的微血管密度。比较了不同组织学亚型、分期和患者预后的肿瘤中的微血管密度。检查CD31免疫染色切片，选择微血管密度高和平均的区域。在CD34和VIII因子相关抗原免疫染色的连续切片上定位相同区域，获得微血管计数并换算为每平方毫米血管数（mm⁻²）。与浆液性肿瘤（105.4±20.7；33.3±6.8）和良性肿瘤（84.4±19.4；20.4±4.4）相比，CD31和CD34免疫染色显示黏液性肿瘤的高血管密度区域（222.4±24.8）和平均血管密度区域（79.9±8.5）的微血管密度均增加（P<0.001）。CD31和CD34免疫染色还显示，早期黏液性肿瘤（234.6±28.2；87.8±9.2）的微血管密度高于早期浆液性肿瘤（72.8±15；12.9±2.4）和晚期浆液性肿瘤（115.6±26.5；29.8±8.5）（P<0.001）。在不同预后患者的样本中未观察到微血管密度的差异（P>0.05）。在交界性和恶性黏液性及浆液性肿瘤中，与CD31和CD34免疫染色相比，VIII因子相关抗原减少（P<0.02），但在良性肿瘤中无此现象（P>0.05）。我们的结果与微血管密度增加和预后不良之间的假定关联相矛盾，并表明卵巢肿瘤类型之间血管生成的水平和调控可能不同。
