Department of Urology, Guizhou Provincial People's Hospital, Guiyang, Guizhou, People's Republic of China.
Institute of Medical Microbiology and Hygiene, Medical Center University of Freiburg, Freiburg, Germany.
Eur J Med Res. 2022 Jun 7;27(1):87. doi: 10.1186/s40001-022-00719-7.
Currently, there are relatively few studies on the effects of changes in oestrogen and androgen levels on prostatic microvessel density (MVD). This article aimed to study the changes in prostatic MVD in Sprague-Dawley (SD) rats after castration under the effect of oestrogen/androgen at different concentrations.
Male SD rats aged 3-4 months were randomly divided into a control group, a castration group, and groups with different concentrations of oestrogen/androgen treatment after castration. Dihydrotestosterone (DHT) and oestradiol (E) were administered daily by subcutaneous injection for one month. All the rats were killed by cervical dislocation after one month, and the serum DHT and E concentrations of the rats in each group were measured by ELISA. Prostate tissue specimens were immunohistochemically stained with monoclonal antibodies against CD34 and factor VIII for MVD.
Compared with the control group, the MVD decreased significantly in the castration group (P < 0.05). When the exogenous E concentration was constant, in general, the MVD of rats in all the groups increased with increasing exogenous DHT concentration. Compared with the castration group, the MVD increased significantly in the E0.05 + DHT0.015 mg/kg, E0.05 + DHT0.05 mg/kg, E0.05 + DHT0.15 mg/kg, E0.05 + DHT0.5 mg/kg, and E0.05 + DHT1.5 mg/kg groups (P < 0.05). In addition, when the exogenous DHT concentration was constant, the MVD increased with increasing exogenous E concentration in all the groups. Among them, compared with the control and castration groups, the MVD increased significantly in the DHT0.15 + E0.015 mg/kg, DHT0.15 + E0.15 mg/kg, and DHT0.15 + E0.5 mg/kg groups (P < 0.05).
Androgens play an important role in the regulation of prostatic MVD in SD rats, and a decrease in DHT concentration can induce a decrease in prostatic MVD. In contrast, prostatic MVD can be increased with increasing DHT concentration. In addition, prostatic MVD can be increased gradually with increasing oestrogen concentration.
目前，关于雌激素和雄激素水平变化对前列腺微血管密度（MVD）的影响的研究相对较少。本文旨在研究不同浓度的雌激素/雄激素作用下，去势雄性 SD 大鼠前列腺 MVD 的变化。
将 3-4 月龄雄性 SD 大鼠随机分为对照组、去势组和去势后不同浓度雌激素/雄激素处理组。通过皮下注射二氢睾酮（DHT）和雌二醇（E），每天给药 1 个月。1 个月后，所有大鼠均通过颈椎脱位处死，通过 ELISA 测量每组大鼠的血清 DHT 和 E 浓度。用抗 CD34 和因子 VIII 单克隆抗体对前列腺组织标本进行免疫组织化学染色，以检测 MVD。
与对照组相比，去势组的 MVD 显著降低（P<0.05）。当外源性 E 浓度保持不变时，一般来说，所有组中大鼠的 MVD 随着外源性 DHT 浓度的增加而增加。与去势组相比，E0.05+DHT0.015mg/kg、E0.05+DHT0.05mg/kg、E0.05+DHT0.15mg/kg、E0.05+DHT0.5mg/kg 和 E0.05+DHT1.5mg/kg 组的 MVD 显著增加（P<0.05）。此外，当外源性 DHT 浓度保持不变时，所有组中 MVD 随着外源性 E 浓度的增加而增加。其中，与对照组和去势组相比，DHT0.15+E0.015mg/kg、DHT0.15+E0.15mg/kg 和 DHT0.15+E0.5mg/kg 组的 MVD 显著增加（P<0.05）。
雄激素在调节 SD 大鼠前列腺 MVD 中起重要作用，DHT 浓度的降低可导致前列腺 MVD 降低。相反，前列腺 MVD 可随 DHT 浓度的增加而增加。此外，前列腺 MVD 可随雌激素浓度的增加而逐渐增加。
