Fish Health Management Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar-2, Orissa, India.
Fish Shellfish Immunol. 2009 Dec;27(6):678-83. doi: 10.1016/j.fsi.2009.08.002. Epub 2009 Aug 21.
The present experiment was carried out to study the effect of different dosages of beta-glucan suspension derived from barley on the innate immune response and disease resistance of Anabas testudineus spawns against infection caused by Aeromonas hydrophila. Four different dosages of beta-glucan suspension in phosphate buffered saline at the rate of 0, 5, 10, 15 mg l(-1) were taken and 8 days old spawn were exposed for 2 h and 3 h. The cell suspension of spawn was assayed for total protein, acid phosphatase activity, lysozyme activity, bactericidal and NBT. Further, the spawns were challenged with 3 x 10(5) cells ml(-1) of A. hydrophila and survivability percentage and immunological parameters were assayed upto day 7. On day 7, most of the immunological parameters such as lysozyme activity, bactericidal activity and NBT activity were significantly enhanced after exposing the fish to all the concentrations of beta-glucan. Challenge study indicated least mortality in the group of spawns immersed in 15 mg l(-1) beta-glucan suspension for 3 h. Thus, 3 h exposure to beta-glucan suspension could reduce the mortality and increase the immunity of A. testudineus spawns.
本实验旨在研究不同剂量的大麦β-葡聚糖悬浮液对安汶对虾幼体先天免疫反应和抗嗜水气单胞菌感染能力的影响。采用磷酸盐缓冲液中的四种不同剂量的β-葡聚糖悬浮液，浓度分别为 0、5、10、15mg/L，将 8 天大的幼虾暴露于其中 2 小时和 3 小时。检测幼虾细胞悬浮液中的总蛋白、酸性磷酸酶活性、溶菌酶活性、杀菌活性和 NBT。进一步，用 3×10(5)个细胞/ml 的嗜水气单胞菌对幼虾进行攻毒，在第 7 天检测存活率和免疫参数。第 7 天，暴露于所有浓度β-葡聚糖的幼虾的溶菌酶活性、杀菌活性和 NBT 活性等大多数免疫参数显著增强。攻毒研究表明，在 15mg/Lβ-葡聚糖悬浮液中浸泡 3 小时的幼虾死亡率最低。因此，3 小时暴露于β-葡聚糖悬浮液可以降低安汶对虾幼体的死亡率并提高其免疫力。
