Misra C K, Das B K, Mukherjee S C, Meher P K
Aquatic Animal Health Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar-2, Orissa 751 002, India.
Fish Shellfish Immunol. 2006 May;20(5):728-38. doi: 10.1016/j.fsi.2005.09.004. Epub 2005 Nov 15.
The purpose of this study was to determine if injections of different dosages of tuftsin would enhance the immune response and disease resistance against the infections due to the opportunistic pathogens Aeromonas hydrophila and Edwardsiella tarda in Labeo rohita fingerlings. Hence, four different dosages of tuftsin in PBS suspension at the rate of 0, 5, 10, 15 mg kg(-1) body weight of fish were injected intraperitoneally to the fingerlings of L. rohita at 2-week intervals for four times. After every 2-week interval, different serum biochemical, haematological and immunological parameters of fish were evaluated. Biochemical and haematological parameters including serum total protein content, albumin content, globulin content, albulin:globulin ratio, glucose content, leucocyte counts etc.; cellular immune parameters including superoxide anion production, phagocytic activities, lymphokine production index etc.; humoral immune parameters including lysozyme activity, complement activity, serum bactericidal activity etc., in the fish were evaluated after every 2-week interval. After 56 days, fish were divided into two subgroups under each major treatment group for challenge with two pathogens A. hydrophila and E. tarda. The mortality (%) and agglutinating antibody titre was recorded on 28th day post challenge. Most of the immune parameters including leucocyte count, phagocytic ratio, phagocytic index, lysozyme activity, complement activity, and serum bactericidal activity were significantly (p<or=0.05) maximum on 42 days after three i.p. injections of 10 mg kg(-1) body weight of tuftsin. Challenge study indicated least mortality in the group of fish injected with 10 mg kg(-1) body weight of tuftsin for four times. Multiple injections of tuftsin might have maintained the activation of phagocytic cells for a long period, which in turn led to long-term protection in the fish. Thus, multiple injections of 10 mg kg(-1) body weight of tuftsin for three times can be advocated for enhancing the immune response of fish species under aquaculture.
本研究的目的是确定注射不同剂量的促吞噬素是否会增强印度鲤鱼幼鱼对嗜水气单胞菌和迟缓爱德华氏菌等机会致病菌感染的免疫反应和抗病能力。因此，以0、5、10、15 mg kg(-1)鱼体重的比例，将促吞噬素在PBS悬浮液中的四种不同剂量，每隔2周腹腔注射到印度鲤鱼幼鱼体内，共注射4次。每隔2周，评估鱼的不同血清生化、血液学和免疫学参数。每隔2周评估鱼的生化和血液学参数，包括血清总蛋白含量、白蛋白含量、球蛋白含量、白蛋白:球蛋白比值、葡萄糖含量、白细胞计数等；细胞免疫参数，包括超氧阴离子产生、吞噬活性、淋巴因子产生指数等；体液免疫参数，包括溶菌酶活性、补体活性、血清杀菌活性等。56天后，在每个主要治疗组下将鱼分为两个亚组，用嗜水气单胞菌和迟缓爱德华氏菌两种病原体进行攻毒。在攻毒后第28天记录死亡率(%)和凝集抗体效价。在腹腔注射10 mg kg(-1)体重的促吞噬素3次后，大多数免疫参数，包括白细胞计数、吞噬率、吞噬指数、溶菌酶活性、补体活性和血清杀菌活性，在42天时显著(p≤0.05)最高。攻毒研究表明，四次注射10 mg kg(-1)体重促吞噬素的鱼组死亡率最低。多次注射促吞噬素可能长期维持吞噬细胞的活化，进而在鱼体内产生长期保护作用。因此，可提倡三次腹腔注射10 mg kg(-1)体重的促吞噬素，以增强水产养殖鱼类的免疫反应。
