Misra Chandra Kanta, Das Basanta Kumar, Mukherjee Subhash Chandra, Pattnaik Phalguni
Central Institute of Fisheries Education, Fisheries University Road, Seven Bungalows, Andheri (W), Mumbai 400 061, India.
Fish Shellfish Immunol. 2006 Mar;20(3):305-19. doi: 10.1016/j.fsi.2005.05.007.
The purpose of this study was to determine if multiple injections of different dosages of beta-glucan derived from barley would enhance the immune response and disease resistance against infections due to opportunistic pathogens Aeromonas hydrophila and Edwardsiella tarda in Labeo rohita fingerlings. Hence, four different dosages of beta-glucan suspension in phosphate-buffered saline at the rate of 0, 5, 10, 15 mg kg(-1) body weight of fish were injected intraperitoneally to the fingerlings of Labeo rohita at two-week intervals for four times. After every two-week interval different serum biochemical, haematological and immunological parameters of fish were evaluated. At the end of immunostimulation trial of 56 days, fish were divided into four subgroups under each major treatment group for challenge through i.p injection and bath immersion with two pathogens, A. hydrophila and E. tarda. The mortality (%) and agglutinating antibody titre was recoded on 28th day post challenge. Most of the immune parameters such as leucocyte count, phagocytic ratio, phagocytic index, lysozyme activity, complement activity, serum bactericidal activity were significantly (P < 0.05) enhanced on 42 days after three i.p injections of 10 mg of beta-glucan kg(-1) body wt. Challenge study indicated least mortality in the group of fishes injected with medium dose of 10 mg of beta-glucan kg(-1) body wt. four times. Multiple injections of beta-glucan might have maintained the activation of phagocytic cells for a long period which in turn would lead to long-term protection in fishes. Thus, injections of 10 mg of beta-glucan kg(-1) body wt. for three times can be advocated to enhance the immune response of fish species under aquaculture.
本研究的目的是确定多次注射不同剂量的源自大麦的β-葡聚糖是否会增强印度鲤鱼幼鱼对嗜水气单胞菌和迟缓爱德华氏菌等机会性病原体感染的免疫反应和抗病能力。因此，以0、5、10、15mg kg(-1)鱼体重的剂量将β-葡聚糖悬浮于磷酸盐缓冲盐水中，每隔两周对印度鲤鱼幼鱼进行一次腹腔注射，共注射四次。每隔两周对鱼的不同血清生化、血液学和免疫学参数进行评估。在56天的免疫刺激试验结束时，将每个主要治疗组的鱼分为四个亚组，通过腹腔注射和浸浴两种病原体嗜水气单胞菌和迟缓爱德华氏菌进行攻毒。在攻毒后第28天记录死亡率（%）和凝集抗体效价。在腹腔注射三次10mgβ-葡聚糖kg(-1)体重后42天，大多数免疫参数如白细胞计数、吞噬率、吞噬指数、溶菌酶活性、补体活性、血清杀菌活性均显著（P<0.05）增强。攻毒研究表明，注射中等剂量10mgβ-葡聚糖kg(-1)体重四次的鱼组死亡率最低。多次注射β-葡聚糖可能长期维持吞噬细胞的活化，进而为鱼类提供长期保护。因此，提倡腹腔注射三次10mgβ-葡聚糖kg(-1)体重以增强水产养殖鱼类的免疫反应。
