Zhang Rong, Liberski Albert, Sanchez-Martin Rosario, Bradley Mark
School of Chemistry, Kings Building, West Mains Road, University of Edinburgh, Edinburgh EH9 3JJ, UK.
Biomaterials. 2009 Oct;30(31):6193-201. doi: 10.1016/j.biomaterials.2009.07.055. Epub 2009 Aug 22.
In this paper we describe an approach whereby over 2000 individual polymers were synthesized, in situ, on a microscope slide using inkjet printing. Subsequent biological analysis of the entire library allowed the rapid identification of specific polymers with the desired properties. Herein we demonstrate how this array of new materials could be used for the identification of polymers that allow cellular adherence, proliferation and then mild thermal release, for multiple cell lines, including mouse embryonic stem (mES) cells. The optimal, identified hydrogels were successfully scaled-up and demonstrated excellent cell viability after thermal detachment for all cell lines tested. We believe that this approach offers an avenue to the discovery of a specific thermal release polymer for every cell line.
在本文中，我们描述了一种方法，通过该方法使用喷墨打印在显微镜载玻片上原位合成了2000多种单个聚合物。随后对整个文库进行生物学分析，从而能够快速鉴定出具有所需特性的特定聚合物。在此，我们展示了如何将这种新材料阵列用于鉴定多种细胞系（包括小鼠胚胎干细胞（mES））中能够实现细胞黏附、增殖并随后温和热释放的聚合物。所鉴定出的最佳水凝胶成功实现了放大生产，并在热解离后对所有测试细胞系均表现出优异的细胞活力。我们相信，这种方法为发现适用于每种细胞系的特定热释放聚合物提供了一条途径。
