Oskoueian Ehsan, Abdullah Norhani, Hendra Rudi, Karimi Ehsan
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia (UPM), 43400 UPM Serdang, Selangor, Malaysia.
Int J Mol Sci. 2011;12(12):8610-25. doi: 10.3390/ijms12128610. Epub 2011 Nov 29.
Evaluation of abundantly available agro-industrial by-products for their bioactive compounds and biological activities is beneficial in particular for the food and pharmaceutical industries. In this study, rapeseed meal, cottonseed meal and soybean meal were investigated for the presence of bioactive compounds and antioxidant, anti-inflammatory, xanthine oxidase and tyrosinase inhibitory activities. Methanolic extracts of rapeseed meal showed significantly (P < 0.01) higher phenolics and flavonoids contents; and significantly (P < 0.01) higher DPPH and nitric oxide free radical scavenging activities when compared to that of cottonseed meal and soybean meal extracts. Ferric thiocyanate and thiobarbituric acid tests results showed rapeseed meal with the highest antioxidant activity (P < 0.01) followed by BHT, cotton seed meal and soybean meal. Rapeseed meal extract in xanthine oxidase and tyrosinase inhibitory assays showed the lowest IC(50) values followed by cottonseed and soybean meals. Anti-inflammatory assay using IFN-γ/LPS stimulated RAW 264.7 cells indicated rapeseed meal is a potent source of anti-inflammatory agent. Correlation analysis showed that phenolics and flavonoids were highly correlated to both antioxidant and anti-inflammatory activities. Rapeseed meal was found to be promising as a natural source of bioactive compounds with high antioxidant, anti-inflammatory, xanthine oxidase and tyrosinase inhibitory activities in contrast to cotton and soybean meals.
评估大量可得的农用工业副产品中的生物活性化合物及其生物活性，对食品和制药行业尤其有益。在本研究中，对菜籽粕、棉籽粕和大豆粕中的生物活性化合物以及抗氧化、抗炎、黄嘌呤氧化酶和酪氨酸酶抑制活性进行了研究。与棉籽粕和大豆粕提取物相比，菜籽粕的甲醇提取物显示出显著更高（P < 0.01）的酚类和黄酮类化合物含量，以及显著更高（P < 0.01）的DPPH和一氧化氮自由基清除活性。硫氰酸铁和硫代巴比妥酸测试结果表明，菜籽粕的抗氧化活性最高（P < 0.01），其次是丁基羟基甲苯、棉籽粕和大豆粕。在黄嘌呤氧化酶和酪氨酸酶抑制试验中，菜籽粕提取物的IC(50)值最低，其次是棉籽粕和大豆粕。使用IFN-γ/LPS刺激的RAW 264.7细胞进行的抗炎试验表明，菜籽粕是一种有效的抗炎剂来源。相关性分析表明，酚类和黄酮类化合物与抗氧化和抗炎活性高度相关。与棉籽粕和大豆粕相比，菜籽粕有望成为具有高抗氧化、抗炎、黄嘌呤氧化酶和酪氨酸酶抑制活性的生物活性化合物的天然来源。
