Scientific Institute of Pavia, Italy.
Int Arch Occup Environ Health. 2010 Feb;83(2):217-23. doi: 10.1007/s00420-009-0459-9. Epub 2009 Aug 23.
To investigate the usefulness of nasal blown secretion evaluation during specific nasal provocation test (sNPT) in diagnosing occupational rhinitis (OR).
To validate the method, nasal blown secretions from 103 healthy subjects and 30 allergic rhinitis patients were analyzed. Furthermore, we analyzed nasal blown secretions, collected before and after sNPT, of 29 subjects with work-related rhinitis symptoms (WRRS) who underwent the diagnostic pathway for OR. Rhinoscopy and nasal symptom score were used to define a positive sNPT.
A total of 89.6% WRRS subjects provided suitable nasal secretions. Eosinophils significantly increased after positive sNPTs compared to negative ones (P = 0.006). Four percent and/or 1 x 10(4) eosinophils/ml was the cut-off for a significant post-sNPT eosinophil increase. A total of 4/13 (33%) WRRS subjects with negative sNPT, assessed by rhinoscopy and nasal symptom score, presented a significant post-sNPT nasal eosinophil increase, and were identified as possible OR.
Eosinophil evaluation in nasal blown secretions is an important tool in monitoring the response to occupational specific nasal challenges.
研究在特定鼻激发试验（sNPT）期间评估鼻吹分泌物对职业性鼻炎（OR）的诊断价值。
为了验证该方法，分析了 103 名健康受试者和 30 名变应性鼻炎患者的鼻吹分泌物。此外，我们还分析了 29 例有工作相关鼻炎症状（WRRS）并接受 OR 诊断途径的受试者的 sNPT 前后采集的鼻吹分泌物。鼻镜检查和鼻部症状评分用于定义阳性 sNPT。
共 89.6%的 WRRS 受试者提供了合适的鼻分泌物。与阴性 sNPT 相比，阳性 sNPT 后嗜酸性粒细胞明显增加（P = 0.006）。4%和/或 1 x 10(4)嗜酸性粒细胞/ml 为 sNPT 后嗜酸性粒细胞显著增加的截止值。在通过鼻镜检查和鼻部症状评分评估的 13 名 WRRS 受试者中，有 4 名（33%）阴性 sNPT 受试者出现了显著的 sNPT 后鼻内嗜酸性粒细胞增加，并被确定为可能的 OR。
鼻吹分泌物中嗜酸性粒细胞的评估是监测对职业性特异性鼻挑战反应的重要工具。
