Department of Oral Biology, Medical College of Georgia, Augusta, Georgia, USA.
J Biomed Mater Res A. 2010 Jun 1;93(3):864-9. doi: 10.1002/jbm.a.32407.
Titanates are inorganic compounds with high affinity for specific metal ions or metal compounds, including gold. We have previously demonstrated that both monosodium titanate (MST) and amorphous peroxo-titanate (APT) alone do not suppress cellular metabolism of several cell types, and we have shown that MST and APT adsorb and release gold compounds in biological salt solutions. In the current study, we extend this work and show that MST and APT loaded with two gold compounds deliver sufficient levels of these compounds to alter the metabolism of mammalian cells. Fibroblasts (L929) or monocytes (THP1) were exposed to MST and APT loaded with either Au(III) or Auranofin(R), a Au(I)-organic compound, for 24-72 h, after which succinate dehydrogenase (SDH) activity of the cells was measured using the MTT method. MST or APT alone did not suppress SDH activity of either cell type. AF and Au(III) alone suppressed SDH activity completely above 2 muM or 300 muM, respectively. APT and MST loaded with either gold compound suppressed L929 fibroblast SDH activity by 30-80% after 72 h, but Au(III)-loaded APT was more potent than AF-loaded APT. Monocyte SDH activity was not affected by any loaded titanate. Our results suggest that titanates could be used for solid phase delivery of metal compounds to affect mammalian cell function of some types of cells.
钛酸盐是一种对特定金属离子或金属化合物（包括金）具有高亲和力的无机化合物。我们之前已经证明，单 2-钠 钛酸盐 (MST) 和无定形过氧钛酸盐 (APT) 单独使用并不会抑制几种细胞类型的细胞代谢，并且我们已经表明 MST 和 APT 可以在生物盐溶液中吸附和释放金化合物。在当前的研究中，我们扩展了这项工作，并表明负载有两种金化合物的 MST 和 APT 可以将这些化合物输送到足够的水平，从而改变哺乳动物细胞的代谢。将 MST 和 APT 负载有 Au(III) 或 Auranofin(R)（一种 Au(I)-有机化合物）的成纤维细胞 (L929) 或单核细胞 (THP1) 暴露于其中 24-72 h，然后使用 MTT 法测量细胞中的琥珀酸脱氢酶 (SDH) 活性。单独的 MST 或 APT 不会抑制两种细胞类型的 SDH 活性。AF 和 Au(III) 单独使用时，其浓度分别超过 2 μM 或 300 μM 时，完全抑制 SDH 活性。负载有金化合物的 APT 和 MST 在 72 h 后可使 L929 成纤维细胞的 SDH 活性降低 30-80%，但负载 Au(III)的 APT 比负载 AF 的 APT 更有效。负载钛酸盐对单核细胞的 SDH 活性没有影响。我们的结果表明，钛酸盐可用于金属化合物的固相输送，以影响某些类型细胞的哺乳动物细胞功能。
