Chen Yen-Wei, Drury Jeanie L, Moussi Joelle, Taylor-Pashow Kathryn M L, Hobbs David T, Wataha John C
Department of Restorative Dentistry, University of Washington School of Dentistry, Seattle, WA 98195-7456, USA.
Savannah River National Laboratory, Aiken, SC 29808, USA.
Biomed Res Int. 2016;2016:7895182. doi: 10.1155/2016/7895182. Epub 2016 Dec 1.
Monosodium titanates (MST) are a relatively novel form of particulate titanium dioxide that have been proposed for biological use as metal sorbents or delivery agents, most recently calcium (II). In these roles, the toxicity of the titanate or its metal complex is crucial to its biological utility. The aim of this study was to determine the cytotoxicity of MST and MST-calcium complexes with MC3T3 osteoblast-like cells; MST-Ca(II) complexes could be useful to promote bone formation in various hard tissue applications. MC3T3 cells were exposed to native MST or MST-Ca(II) complexes for 24-72 h. A CellTiter-Blue® assay was employed to assess the metabolic activity of the cells. The results showed that MST and MST-Ca(II) suppressed MC3T3 metabolic activity significantly in a dose-, time-, and cell-density-dependent fashion. MST-Ca(II) suppressed MC3T3 metabolism in a statistically identical manner as native MST at all concentrations. We concluded that MST and MST-Ca(II) are significantly cytotoxic to MC3T3 cells through a mechanism yet unknown; this is a potential problem to the biological utility of these complexes.
钛酸钠（MST）是一种相对新型的颗粒状二氧化钛，最近被提议用作生物金属吸附剂或递送剂，尤其是钙（II）。在这些应用中，钛酸盐或其金属络合物的毒性对其生物学效用至关重要。本研究的目的是确定MST和MST-钙络合物对MC3T3成骨样细胞的细胞毒性；MST-Ca（II）络合物可能有助于在各种硬组织应用中促进骨形成。将MC3T3细胞暴露于天然MST或MST-Ca（II）络合物中24至72小时。采用CellTiter-Blue® 检测法评估细胞的代谢活性。结果表明，MST和MST-Ca（II）以剂量、时间和细胞密度依赖性方式显著抑制MC3T3代谢活性。在所有浓度下，MST-Ca（II）对MC3T3代谢的抑制作用在统计学上与天然MST相同。我们得出结论，MST和MST-Ca（II）通过一种尚不清楚的机制对MC3T3细胞具有显著的细胞毒性；这对这些络合物的生物学效用来说是一个潜在问题。
