中药对离体灌注大鼠下颌下腺唾液分泌的影响。

Effects of Chinese herbs on salivary fluid secretion by isolated and perfused rat submandibular glands.

作者信息

Murakami Masataka, Wei Mu-Xin, Ding Wei, Zhang Qian-De

机构信息

Nano structure Physiology, National Institute of Physiological Science, Okazaki 444-8585, Aichi, Japan.

出版信息

World J Gastroenterol. 2009 Aug 21;15(31):3908-15. doi: 10.3748/wjg.15.3908.

DOI:10.3748/wjg.15.3908

PMID:19701971

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2731253/

Abstract

AIM

To determine whether Chinese herbs (CHs) relieve xerostomia (dry mouth) by increasing salivary secretion.

METHODS

The submandibular glands of Wistar rats were surgically isolated and perfused arterially with buffered salt solution. After control perfusion, recording started 5 min prior to the start of stimulation. After fluid secretion was induced by 0.2 mumol/L carbamylcholine (CCh) in the perfusate for 10 min, Chinese herb (CH) was added in the perfusion for 5 min. CCh was then overloaded at 0.2 mumol/L in the perfusion for 20 min. The volume of salivary fluid secretion was recorded by a computer-controlled balance system.

RESULTS

Saliva secretion formed an initial ephemeral peak at 30 s followed by a gradual increase to a sustained level. CH alone induced no or little saliva in all types of CH selected. During perfusion with CH, overloading of CCh promoted fluid secretion in 15 of 20 CHs. This promotion was classified into four patterns, which were eventually related to the categories of CH: Overall sustained phase was continuously raised (Yin-nourishing, fluid production-promoting and heat-clearing agents); The sustained secretion rose to reach a maximum then decreased (Qi-enhancing agent); Sustained secretion rose to reach the highest maximum and was then sustained with a slight decline (swelling-reducing, phlegm-resolving and pus-expelling agents); Stimulation of salivary secretion without any added stimulants. Addition of CCh raised the fluid secretion to reach the highest maximum then sharply decreased to a lower sustained level (blood activating agent).

CONCLUSION

The present findings lead to the conclusion that various CHs have different promotional effects directly on the salivary gland.

摘要

目的

确定中药是否通过增加唾液分泌来缓解口干症。

方法

手术分离Wistar大鼠的下颌下腺，并用缓冲盐溶液进行动脉灌注。对照灌注后，在刺激开始前5分钟开始记录。在用0.2 μmol/L氨甲酰胆碱（CCh）诱导灌注液中的液体分泌10分钟后，加入中药灌注5分钟。然后在灌注中以0.2 μmol/L的剂量过量加入CCh 20分钟。唾液分泌量由计算机控制的平衡系统记录。

结果

唾液分泌在30秒时形成一个初始的短暂峰值，随后逐渐增加至持续水平。在所选择的所有类型的中药中，单独使用中药诱导的唾液分泌无或极少。在中药灌注期间，20种中药中有15种在CCh过量加入时促进了液体分泌。这种促进作用分为四种模式，最终与中药类别相关：整个持续阶段持续升高（滋阴、生津和清热剂）；持续分泌先上升至最大值然后下降（补气剂）；持续分泌上升至最高最大值，然后持续并略有下降（消肿、化痰和排脓剂）；在无任何添加刺激物的情况下刺激唾液分泌。加入CCh后，液体分泌先上升至最高最大值，然后急剧下降至较低的持续水平（活血剂）。