Université de Lyon, F-69622, Lyon, France.
Atherosclerosis. 2010 Feb;208(2):467-72. doi: 10.1016/j.atherosclerosis.2009.07.049. Epub 2009 Aug 3.
To determine the effect of supplementation with increasing doses of docosahexaenoic acid (DHA), as the only n-3 polyunsaturated fatty acid (PUFA), on low-density lipoprotein (LDL) redox status and oxidizability.
Twelve healthy men aged 53-65 years ingested consecutive doses of DHA (200, 400, 800 and 1600 mg/day), each dose for two weeks.
The proportions of DHA increased dose-dependently in LDL phospholipids and cholesteryl esters, even after two weeks of supplementation with 200mg/day DHA. The daily intake of 200, 400 and 800 mg DHA resulted in increased alpha-tocopherol concentrations, decreased MDA concentrations, and a longer lag time for copper-induced LDL oxidation. Supplementation with 1600 mg/day DHA had no effect on the above parameters. In plasma, concentrations of 4-hydroxy-hexenal, specifically derived from the peroxidation of n-3 fatty acids, significantly increased after 800 and 1600 mg DHA, representing 0.01% of plasma n-3 PUFAs, while 4-hydroxy-nonenal concentrations, derived from the peroxidation of n-6 fatty acids, did not change.
Our results clearly show that an intake of 200-800 mg/day DHA may have protective and antioxidant effects on LDL and could represent optimal doses for cardiovascular disease prevention in a healthy population.
确定补充二十二碳六烯酸（DHA）作为唯一的 n-3 多不饱和脂肪酸（PUFA）对低密度脂蛋白（LDL）氧化还原状态和氧化性的影响。
12 名年龄在 53-65 岁的健康男性连续摄入 DHA（200、400、800 和 1600mg/天），每种剂量持续两周。
即使在每天补充 200mgDHA 的两周后，LDL 磷脂和胆固醇酯中的 DHA 比例也呈剂量依赖性增加。每天摄入 200、400 和 800mgDHA 会导致α-生育酚浓度增加、MDA 浓度降低，以及铜诱导 LDL 氧化的滞后时间延长。每天补充 1600mgDHA 对上述参数没有影响。在血浆中，4-羟基-己烯醛的浓度（特别是由 n-3 脂肪酸的过氧化产生的）在摄入 800 和 1600mgDHA 后显著增加，代表血浆 n-3PUFA 的 0.01%，而 4-羟基-壬烯醛的浓度（由 n-6 脂肪酸的过氧化产生的）没有变化。
我们的研究结果清楚地表明，每天摄入 200-800mgDHA 可能对 LDL 具有保护和抗氧化作用，并且可以作为健康人群预防心血管疾病的最佳剂量。
