Brown C P, Bowden J C, Rintoul L, Meder R, Oloyede A, Crawford R W
School of Engineering Systems, IHBI, QUT, Brisbane, Australia.
Phys Med Biol. 2009 Sep 21;54(18):5579-94. doi: 10.1088/0031-9155/54/18/015. Epub 2009 Aug 28.
A non-destructive, diffuse reflectance near infrared spectroscopy (DR-NIRS) approach is considered as a potential tool for determining the component-level structural properties of articular cartilage. To this end, DR-NIRS was applied in vitro to detect structural changes, using principal component analysis as the statistical basis for characterization. The results show that this technique, particularly with first-derivative pretreatment, can distinguish normal, intact cartilage from enzymatically digested cartilage. Further, this paper establishes that the use of DR-NIRS enables the probing of the full depth of the uncalcified cartilage matrix, potentially allowing the assessment of degenerative changes in joint tissue, independent of the site of initiation of the osteoarthritic process.
一种非破坏性的漫反射近红外光谱（DR-NIRS）方法被认为是确定关节软骨成分水平结构特性的潜在工具。为此，将DR-NIRS应用于体外检测结构变化，使用主成分分析作为表征的统计基础。结果表明，该技术，特别是经过一阶导数预处理后，能够区分正常、完整的软骨和酶消化后的软骨。此外，本文证实，使用DR-NIRS能够探测未钙化软骨基质的全层深度，这有可能独立于骨关节炎进程起始部位来评估关节组织的退行性变化。
