Karahan Ozlem
Istanbul Technical University, Environmental Engineering Department, Maslak, Istanbul TR-34469, Turkey.
Bioresour Technol. 2010 Jan;101(1):92-7. doi: 10.1016/j.biortech.2009.07.088. Epub 2009 Aug 31.
The study presents a conceptual approach for the identification of the inhibition mechanisms of biodegradable inhibitors. Synthetic sewage was selected as the model degradable substrate to simulate domestic wastewaters. LAS, known to be a biodegradable but inhibitory compound, was selected as a model substrate for the determination of the inhibition mechanisms. Biodegradation of synthetic sewage and LAS were monitored through oxygen uptake rate (OUR) tests conducted to observe the dynamic response of the system when fed with synthetic sewage and synthetic sewage-LAS mixtures. The approach uses respirometry to calibrate the kinetic and stoichiometric coefficients of the proposed biochemical model. Model simulation results confirmed that presence of LAS has inhibitory effects on the biodegradation mechanisms of synthetic sewage. LAS imposed non-competitive inhibition on the hydrolysis process with an inhibition coefficient of 500 mg COD/L and effected heterotrophic growth through a competitive inhibition mechanism with an inhibition coefficient of 150 mg COD/L.
该研究提出了一种用于识别可生物降解抑制剂抑制机制的概念方法。选择合成污水作为模拟生活污水的模型可降解底物。直链烷基苯磺酸盐（LAS）是一种已知的可生物降解但具有抑制性的化合物，被选作确定抑制机制的模型底物。通过进行耗氧速率（OUR）测试来监测合成污水和LAS的生物降解情况，以观察系统在投喂合成污水和合成污水-LAS混合物时的动态响应。该方法利用呼吸测定法来校准所提出生化模型的动力学和化学计量系数。模型模拟结果证实，LAS的存在对合成污水的生物降解机制具有抑制作用。LAS对水解过程施加非竞争性抑制，抑制系数为500 mg COD/L，并通过竞争性抑制机制影响异养生长，抑制系数为150 mg COD/L。
