Le Mouellic H, Lallemand Y, Brûlet P
Unité de Génétique Cellulaire du Collège de France et de l'Institut Pasteur, Paris.
Proc Natl Acad Sci U S A. 1990 Jun;87(12):4712-6. doi: 10.1073/pnas.87.12.4712.
Through gene targeting based upon homologous recombination in embryonic stem cells, a chosen gene can be inactivated and eventually a strain of mutant mice created. We have devised a procedure to specifically replace a targeted gene by another gene. A murine homeobox gene was disrupted at high frequency in embryonic stem cells by its replacement with Escherichia coli lacZ. Injection of such stem cells into blastocysts yielded chimeric embryos in which beta-galactosidase activity was driven by the Hox-3.1 promoter. This technique will allow the visual assessment at the cellular level of gene inactivation effects in transgenic mice.
通过基于胚胎干细胞中同源重组的基因靶向技术，可以使选定的基因失活，并最终培育出突变小鼠品系。我们设计了一种程序，用另一个基因特异性替换目标基因。通过用大肠杆菌lacZ替换，鼠类同源异型盒基因在胚胎干细胞中被高频破坏。将这种干细胞注射到囊胚中产生了嵌合胚胎，其中β-半乳糖苷酶活性由Hox-3.1启动子驱动。这项技术将允许在细胞水平上对转基因小鼠中的基因失活效应进行可视化评估。
