Chen Jun-Qiang, Zhan Wen-Hua, He Yu-Long, Peng Jun-Sheng, Wang Jian-Ping, Cai Shi-Rong, Ma Jin-Ping
Department of Gastrointestinal Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China.
World J Gastroenterol. 2004 Mar 15;10(6):776-82. doi: 10.3748/wjg.v10.i6.776.
To explore the expression of heparanase gene, CD44v6, MMP-7 and nm23 proteins in human gastric carcinoma as well as their relationship with the clinicopathological factors.
Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the expressions of heparanase mRNA in 43 human gastric carcinomas and 10 adjacent normal gastric tissues. Streptavidin-peroxidase (S-P) two step method was used to measure the immunohistochemical expression of CD44v6, MMP-7 and nm23 protein in 43 human gastric carcinomas.
The expression of heparanase gene was positive in 29 cases of gastric cancer with a positive rate of 67.4%, which was significantly higher than those in adjacent normal gastric tissues (P<0.05). The heparanase mRNA expression was significantly related to advanced stage of disease, serosal infiltration, lymph node metastasis and size of tumors (P<0.05), but not related to tumor location, gross and histological types of the cancer, peritoneal dissemination and liver metastasis (P>0.05). The positive rate of CD44v6, nm23 and MMP-7 proteins was 76.7%, 37.2% and 60.5%, respectively. The CD44v6 protein expression was significantly related to serosal infiltration, lymph node metastasis and TNM stage of disease (P<0.05). The nm23 protein expression was significantly related to the tumor gross appearance, lymph node and peritoneal metastasis (P<0.05). The MMP-7 protein expression was significantly related to serosal infiltration and TNM stage of disease (P<0.05). In an attempt to measure the association between these agents, we found that the expression of heparanase mRNA had significantly negative correlation with the expression of CD44v6 and MMP-7 protein (P<0.05), the expression of MMP-7 protein had significantly positive correlation with the expression of CD44v6 protein (r=0.568, P<0.05), the expression of MMP-7 protein had no correlation with the expression of nm23 protein (P>0.05), and the expression of nm23 protein had no correlation with the expression of CD44v6 protein (P>0.05).
Despite their different mechanisms of action, heparanase, CD44v6 and nm23 may play important roles in the invasive infiltration and lymph node metastasis in gastric carcinomas. Instead of metastatic spreading, MMP-7 may be just related to the invasion of gastric carcinomas. However, co-detection of these factors may be important to predict metastatic spreading in such patients.
探讨乙酰肝素酶基因、CD44v6、基质金属蛋白酶-7（MMP-7）及nm23蛋白在人胃癌中的表达及其与临床病理因素的关系。
采用逆转录聚合酶链反应（RT-PCR）检测43例人胃癌组织及10例癌旁正常胃组织中乙酰肝素酶mRNA的表达。采用链霉亲和素-过氧化物酶（S-P）两步法检测43例人胃癌组织中CD44v6、MMP-7及nm23蛋白的免疫组化表达。
29例胃癌组织中乙酰肝素酶基因表达阳性，阳性率为67.4%，显著高于癌旁正常胃组织（P<0.05）。乙酰肝素酶mRNA表达与疾病晚期、浆膜浸润、淋巴结转移及肿瘤大小显著相关（P<0.05），但与肿瘤部位、大体类型、组织学类型、腹膜播散及肝转移无关（P>0.05）。CD44v6、nm23及MMP-7蛋白的阳性率分别为76.7%、37.2%及60.5%。CD44v6蛋白表达与浆膜浸润、淋巴结转移及疾病TNM分期显著相关（P<0.05）。nm23蛋白表达与肿瘤大体形态、淋巴结及腹膜转移显著相关（P<0.05）。MMP-7蛋白表达与浆膜浸润及疾病TNM分期显著相关（P<0.05）。为了检测这些因子之间的关联，我们发现乙酰肝素酶mRNA表达与CD44v6及MMP-7蛋白表达呈显著负相关（P<0.05），MMP-7蛋白表达与CD44v6蛋白表达呈显著正相关（r=0.568，P<0.05），MMP-7蛋白表达与nm23蛋白表达无相关性（P>0.05），nm23蛋白表达与CD44v6蛋白表达无相关性（P>0.05）。
尽管乙酰肝素酶、CD44v6及nm23作用机制不同，但它们可能在胃癌的侵袭浸润及淋巴结转移中发挥重要作用。MMP-7可能仅与胃癌的侵袭有关，而非转移扩散。然而，联合检测这些因子对预测此类患者的转移扩散可能具有重要意义。
