Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Biotechnol Bioeng. 2010 Jan 1;105(1):59-68. doi: 10.1002/bit.22522.
Sessile filter-feeding marine sponges (Porifera) have been reported to possess high efficiency in removing bacteria pollution from natural or aquaculture seawater. However, no investigation has been carried out thus far in a true mariculture farm water system. Therefore this study sought to investigate the ability of the marine sponge Hymeniacidon perlevis to bioremediate the bacteria pollution in the intensive aquaculture water system of turbot Scophthalmus maximus. Sponge specimens were hung in fish culture effluent at different temperature to investigate the optimal temperature condition for bacteria removal by H. perlevis. Turbots S. maximus were co-cultured with sponge H. perlevis in 1.5 m(3) of water system at 15-18 degrees C for 6 weeks to control the growth of bacteria. It was found that H. perlevis was able to remove pathogenic bacteria efficiently at 10-20 degrees C, with a maximal removal of 71.4-78.8% of fecal coliform, 73.9-98.7% of pathogenic vibrio, and 75.0-83.7% of total culturable bacteria from fish-culture effluent at 15 degrees C; H. perlevis continuously showed good bioremediation of bacteria pollution in the S. maximus culture water system, achieving removal of 60.0-90.2% of fecal coliform, 37.6-81.6% of pathogenic vibrio, and 45.1-83.9% of total culturable bacteria. The results demonstrate that H. perlevis is an effective bioremediator of bacteria pollution in the turbot S. maximus culture farm water system.
无柄滤食性海洋海绵（多孔动物门）已被报道具有从自然或水产养殖海水中高效去除细菌污染的能力。然而，迄今为止，在真正的海水养殖农场水系统中尚未进行任何调查。因此，本研究旨在研究海洋海绵 Hymeniacidon perlevis 在大菱鲆 Scophthalmus maximus 集约化水产养殖水系统中生物修复细菌污染的能力。将海绵标本悬挂在不同温度的鱼类养殖废水中，以研究 H. perlevis 去除细菌的最佳温度条件。在 15-18°C 的 1.5 m³水系统中，将大菱鲆 S. maximus 与海绵 H. perlevis 共培养 6 周，以控制细菌的生长。结果发现，H. perlevis 能够在 10-20°C 下有效去除病原菌，在 15°C 时，对养殖废水中粪大肠菌群的去除率最高可达 71.4-78.8%，对致病性弧菌的去除率为 73.9-98.7%，对总可培养细菌的去除率为 75.0-83.7%；H. perlevis 持续对大菱鲆养殖水系统中的细菌污染具有良好的生物修复能力，对粪大肠菌群的去除率为 60.0-90.2%，对致病性弧菌的去除率为 37.6-81.6%，对总可培养细菌的去除率为 45.1-83.9%。结果表明，H. perlevis 是大菱鲆养殖农场水系统中细菌污染的有效生物修复剂。
