Kusugami K, Matsuura T, Kawase T, Ina K, Matsunaga H, Kuwahara Y, Inagaki T, Morise K, Shimokata K
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Gastroenterol Jpn. 1990 Jun;25(3):306-13. doi: 10.1007/BF02779443.
The interleukin 2 (IL-2) and interferon-gamma (IFN-gamma) activities of regional lymph node mononuclear cells (LNMC) were studied in 22 patients with gastric cancer. Regional LNMC could produce high levels of IL-2 and IFN-gamma independent of the disease extension or the location of the lymph nodes. The activities of peripheral blood mononuclear cells (PBMC) from cancer patients were decreased especially in advanced disease compared to controls. The lymph node CD4+ cells were responsible for the bulk of IL-2 activity, whereas both CD4+ and CD8+ cells were equally capable of producing IFN-gamma. The proportion of T cells in LNMC was similar to that seen in PBMC, however, CD4+ cells predominated over CD8+ cells in the lymph nodes, contributing to higher activities of IL-2 and IFN-gamma in LNMC. Furthermore, significant cytotoxic activity could be induced from PBMC using the culture supernatant derived from autologous LNMC in cancer patients. This study suggests that regional lymph nodes are strategically located near the tumor and the local cells are capable of producing high levels of IL-2 and IFN-gamma.
对22例胃癌患者区域淋巴结单个核细胞（LNMC）的白细胞介素2（IL-2）和干扰素-γ（IFN-γ）活性进行了研究。区域LNMC能够产生高水平的IL-2和IFN-γ，与疾病进展或淋巴结位置无关。与对照组相比，癌症患者外周血单个核细胞（PBMC）的活性降低，尤其是在疾病晚期。淋巴结CD4+细胞是IL-2活性的主要来源，而CD4+和CD8+细胞产生IFN-γ的能力相当。LNMC中T细胞的比例与PBMC中的相似，然而，淋巴结中CD4+细胞多于CD8+细胞，这导致LNMC中IL-2和IFN-γ的活性更高。此外，使用癌症患者自体LNMC的培养上清液可诱导PBMC产生显著的细胞毒性活性。本研究表明，区域淋巴结位于肿瘤附近，局部细胞能够产生高水平的IL-2和IFN-γ。
