Laboratory of Cellular Immunology, National Institute of Hygiene, 27, Avenue Ibn Batouta, PB 769, 11400, Rabat, Morocco.
Faculty of Sciences, University Mohammed V Agdal, Rabat, Morocco.
BMC Immunol. 2018 Nov 8;19(1):33. doi: 10.1186/s12865-018-0266-8.
Lymph node tuberculosis (LNTB) is the most frequent extrapulmonary form of tuberculosis (TB). Studies of human tuberculosis at sites of disease are limited. LNTB provides a unique opportunity to compare local in situ and peripheral blood immune response in active Mycobacterium tuberculosis (Mtb) disease. The present study analysed T regulatory cells (Treg) frequency and activation along with CD4+ T cell function in lymph nodes from LNTB patients.
Lymph node mononuclear cells (LNMC) were compared to autologous peripheral blood mononuclear cells (PBMC). LNMC were enriched for CD4+ T cells with a late differentiated effector memory phenotype. No differences were noted in the frequency and mutifunctional profile of memory CD4+ T cells specific for Mtb. The proportion of activated CD4+ and Tregs in LNMC was increased compared to PBMC. The correlation between Tregs and activated CD4+ T cells was stronger in LNMC than PBMC. Tregs in LNMC showed a strong positive correlation with Th1 cytokine production (IL2, IFNγ and TNFα) as well as MIP-1α after Mtb antigen stimulation. A subset of Tregs in LNMC co-expressed HLA-DR and CD38, markers of activation.
Further research will determine the functional relationship between Treg and activated CD4+ T cells at lymph node sites of Mtb infection.
淋巴结结核（LNTB）是最常见的肺外结核（TB）形式。对疾病部位人类结核病的研究是有限的。LNTB 为比较活动性结核分枝杆菌（Mtb）疾病局部原位和外周血免疫反应提供了独特的机会。本研究分析了 LNTB 患者淋巴结中 T 调节细胞（Treg）的频率和激活以及 CD4+T 细胞功能。
将淋巴结单核细胞（LNMC）与自体外周血单核细胞（PBMC）进行比较。LNMC 富含具有晚期分化效应记忆表型的 CD4+T 细胞。针对 Mtb 的记忆 CD4+T 细胞的频率和多功能谱无差异。与 PBMC 相比，LNMC 中激活的 CD4+和 Treg 的比例增加。与 PBMC 相比，LNMC 中 Treg 与激活的 CD4+T 细胞之间的相关性更强。LNMC 中的 Treg 与 Mtb 抗原刺激后 Th1 细胞因子（IL2、IFNγ和 TNFα）以及 MIP-1α的产生呈正相关。LNMC 中 Treg 的一个亚群共表达 HLA-DR 和 CD38，这是激活的标志物。
进一步的研究将确定 Mtb 感染淋巴结部位 Treg 和激活的 CD4+T 细胞之间的功能关系。
