一个新的与发病机制相关的蛋白，LrPR4，来自石蒜属，及其对稻瘟病菌的抗真菌活性。
A new pathogenesis-related protein, LrPR4, from Lycoris radiata, and its antifungal activity against Magnaporthe grisea.
机构信息
Qian Hu Hou Cun 1#, Jiangsu Province Key Laboratory for Plant Ex-Situ Conservation, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, 210014 Nanjing, People's Republic of China.
出版信息
Mol Biol Rep. 2010 Feb;37(2):995-1001. doi: 10.1007/s11033-009-9783-0.
A new Lycoris radiata pathogenesis-related (PR)-4 gene, LrPR4 was isolated. LrPR4 encodes a 142 amino acid protein with a predicted molecular mass of 15.43 kDa and pI of 7.56. The putative LrPR4 shows high similarity to PR4 type proteins from various plant species and belongs to the Barwin family. Like other PR4s from monocot plants, LrPR4 protein contains a conserved Barwin domain and has a signal peptide at its N-terminus. The recombinant LrPR4 protein expressed in Escherichia coli showed activity towards hydrolysing RNA from L. radiata bulbs and antifungal activity. The results of this study suggest that LrPR4 may play a role in the disease resistance responses of plant against pathogen attacks though its antifungal activity.
一个新的石蒜病程相关（PR）-4 基因，LrPR4 被分离出来。LrPR4 编码一个 142 个氨基酸的蛋白质，预测分子量为 15.43 kDa，等电点为 7.56。假定的 LrPR4 与来自各种植物物种的 PR4 型蛋白具有高度相似性，属于 Barwin 家族。与单子叶植物的其他 PR4 一样，LrPR4 蛋白含有一个保守的 Barwin 结构域，在其 N 端有一个信号肽。在大肠杆菌中表达的重组 LrPR4 蛋白对石蒜鳞茎 RNA 具有水解活性和抗真菌活性。本研究结果表明，LrPR4 可能通过其抗真菌活性在植物对病原体攻击的抗病反应中发挥作用。
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