Dahlbäck K, Sakai L
Department of Dermatology, University of Lund, University Hospital, Sweden.
Acta Derm Venereol. 1990;70(3):194-8.
The IgA immunoreactive granules or fibrils, characteristically found in dermal papillae of patients with dermatitis herpetiformis, were previously reported to be associated with microfibrillar bundles. Recently, fibrillin, a component of such 8-12 nm microfibrils, was identified. In normal skin, the fibrillin immunoreactive microfibrils are present at the periphery of elastic fibers and are also present without concomitant amorphous elastin in the dermal papillae close to the lamina densa. The localization of the IgA immunoreactive material in the dermal papillae of 17 patients with dermatitis herpetiformis was compared with the distribution of the fibrillin immunoreactive fiber network. Immunofluorescence methods using FITC- and TRITC-labelled antibodies, an avidin-biotin-peroxidase complex technique, and standard elastin staining procedures, were used in several sequential and double staining procedures. In 13 specimens, in which the IgA reactivity was granular, most of the granules were located at the sites of fibrillin-reactive structures. As it could not be excluded that the collocality was coincidental, it could not be ascertained whether the IgA granules were in fact related to the fibrillin immunoreactive fibers in these specimens. However, in 4 specimens with both granular and fibrillar IgA immunoreactive deposits, these were clearly related to and located at the sites of fibrillin-reactive fibrils in the dermal papillae. The results confirm earlier reports of an association of IgA reactive deposits with microfibrillar bundles in dermatitis herpetiformis skin, though the possibility of their binding to other extracellular matrix component(s) has not been ruled out. The findings suggest that fibrillin may be the structural component (or one of them) to which IgA reactive deposits bind in the skin of patients with dermatitis herpetiformis.
IgA免疫反应性颗粒或原纤维，典型地见于疱疹样皮炎患者的真皮乳头中，此前报道其与微纤维束有关。最近，一种8 - 12纳米微纤维的成分——原纤蛋白被鉴定出来。在正常皮肤中，原纤蛋白免疫反应性微纤维存在于弹性纤维的周边，并且在靠近致密板的真皮乳头中也有存在，且不伴有无定形弹性蛋白。将17例疱疹样皮炎患者真皮乳头中IgA免疫反应性物质的定位与原纤蛋白免疫反应性纤维网络的分布进行了比较。在一系列连续和双重染色过程中，使用了异硫氰酸荧光素（FITC）和四甲基异硫氰酸罗丹明（TRITC）标记抗体的免疫荧光方法、抗生物素蛋白-生物素-过氧化物酶复合物技术以及标准弹性蛋白染色程序。在13个标本中，IgA反应呈颗粒状，大多数颗粒位于原纤蛋白反应性结构的部位。由于不能排除这种共定位是巧合，所以无法确定这些标本中的IgA颗粒是否实际上与原纤蛋白免疫反应性纤维有关。然而，在4个同时有颗粒状和原纤维状IgA免疫反应性沉积物的标本中，这些沉积物明显与真皮乳头中原纤蛋白反应性原纤维的部位相关并位于此处。结果证实了早期关于疱疹样皮炎皮肤中IgA反应性沉积物与微纤维束有关的报道，尽管它们与其他细胞外基质成分结合的可能性尚未排除。这些发现表明，原纤蛋白可能是疱疹样皮炎患者皮肤中IgA反应性沉积物结合的结构成分（或其中之一）。
