Stingl G, Hönigsmann H, Holubar K, Wolff K
J Invest Dermatol. 1976 Oct;67(4):507-12. doi: 10.1111/1523-1747.ep12664537.
A multistep immunocytochemical method utilizing horseradish peroxidase as an immunologically bound marker was used to detect and localize IgA deposits in skin of patients with dermatitis herpetiformis at the ultrastructural level. IgA was found in the upper papillary dermis forming irregular aggregates in seemingly haphazard distribution. These aggregates were associated with microfibrillar bundles and with the microfibrillar component of the elastic tissue. IgA was also detected on anchoring fibrils, but showed no topical relationship to the basal lamina which was always spared. This finding indicates that basal lamina components do not serve as target sites for the immunologic reaction occurring in dermatitis herpetiformis. The selective affinity of IgA deposits to microbfibrillar bundles may be relevant to the hypothesis that the skin pathology in dermatitis herpetiformis is caused by circulating gluten-antigluten complexes, trapped in the skin by reticulin-bound antireticulin antibodies which cross-react with gluten.
采用一种以辣根过氧化物酶作为免疫结合标记物的多步免疫细胞化学方法，在超微结构水平上检测和定位疱疹样皮炎患者皮肤中的IgA沉积物。在真皮乳头上层发现IgA，形成不规则聚集体，分布看似随机。这些聚集体与微原纤维束以及弹性组织的微原纤维成分相关。在锚定原纤维上也检测到了IgA，但与始终未受累的基底层没有局部关系。这一发现表明，基底层成分不是疱疹样皮炎中发生的免疫反应的靶位点。IgA沉积物对微原纤维束的选择性亲和力可能与以下假说相关：疱疹样皮炎的皮肤病理是由循环中的谷蛋白-抗谷蛋白复合物引起的，这些复合物被与谷蛋白交叉反应的网状蛋白结合抗网状蛋白抗体截留在皮肤中。
