INRA, UMR Santé Végétale, Villenave d'Ornon, France.
Pest Manag Sci. 2010 Jan;66(1):35-43. doi: 10.1002/ps.1827.
Management of strawberry powdery mildew, Podopshaera aphanis (Wallr.), requires numerous fungicide treatments. Limiting epidemics is heavily dependent on sterol demethylation inhibitors (DMIs) such as myclobutanil or penconazole. Recently, a noticeable reduction in the efficacy of these triazole fungicides was reported by strawberry growers in France. The goal of this study was to investigate the state of DMI sensitivity of French P. aphanis and provide tools for improved pest management.
Using leaf disc sporulation assays, sensitivity to myclobutanil and penconazole of 23 isolates of P. aphanis was monitored. Myclobutanil EC(50) ranged from less than 0.1 to 14.67 mg L(-1) and for penconazole from 0.04 to 4.2 mg L(-1). A cross-analysis and a Venn diagram showed that there was reduced sensitivity and a positive correlation between the less sensitive myclobutanil and penconazole isolates; 73.9% of isolates were less sensitive to a DMI and 47.8% exhibited less sensitivity to both fungicides.
The results show that sensitivity to myclobutanil and, to a lesser extent, penconazole has become less efficient in strawberry powdery mildew in France. Therefore, urgent action is required in order to document its appearance and optimise methods of control.
草莓白粉病（Podopshaera aphanis）的防治需要多次使用杀菌剂。防治该病的关键在于使用甾醇脱甲基抑制剂（DMIs），例如腈菌唑或咯菌腈。最近，法国草莓种植户报告称，这些三唑类杀菌剂的效果明显降低。本研究的目的是调查法国白粉病菌对 DMI 的敏感性，并提供改进病虫害管理的工具。
采用叶盘孢子萌发试验监测了 23 株白粉病菌对腈菌唑和咯菌腈的敏感性。腈菌唑 EC50 范围为 0.1 至 14.67mg/L，咯菌腈 EC50 范围为 0.04 至 4.2mg/L。交叉分析和韦恩图显示，敏感菌株的敏感性降低，对腈菌唑和咯菌腈的敏感性降低呈正相关；73.9%的分离株对 DMI 的敏感性降低，47.8%的分离株对两种杀菌剂的敏感性降低。
结果表明，法国草莓白粉病菌对腈菌唑的敏感性以及对咯菌腈的敏感性在一定程度上降低。因此，需要紧急采取行动记录其出现情况，并优化防治方法。
