Solá L del Rio, Aceves M, Dueñas A I, González-Fajardo J A, Vaquero C, Crespo M Sanchez, García-Rodríguez C
Vascular Surgery Division, Hospital Clinico Universitario, Valladolid, Spain.
Eur J Vasc Endovasc Surg. 2009 Nov;38(5):635-41. doi: 10.1016/j.ejvs.2009.07.021. Epub 2009 Sep 2.
Leucocyte infiltration in the wall of varicose veins has been reported previously. This study was designed to investigate the expression of pro-inflammatory cytokines and chemokines in control and in patients with varicose veins and to test the effect of treating varicose vein patients with acetylsalicylic acid (ASA) on cytokine expression prior to removal of varices.
Sections of vein were removed during operation from both patient groups, and ribonuclease protection assays (RPAs) were performed to assess the expression of chemokines. Group I included non-varicose saphenous veins from healthy patients undergoing amputation for trauma. Varicose veins were obtained from patients with primary varicose undergoing surgical treatment who received no drug (group II) or treatment with 300 mg day(-1) of ASA for 15 days before surgery (group III).
Non-varicose veins constitutively expressed low levels of monocyte-chemoattractant protein (MCP-1) and interleukin (IL)-8 mRNA. Varicose veins had a distinct chemokine expression pattern, since significant up-regulation of MCP-1 and IL-8 and a marked expression of IP-10, RANTES, MIP-1alpha and MIP-1beta mRNA were detected. Removal of the endothelium did not alter this pattern. Varicose veins obtained from patients treated with ASA showed a consistent decrease in chemokine expression, although it did not reach statistical significance.
Varicose veins showed increased expression of several chemokines compared to control veins. A non-significant reduction of activation was observed following treatment with ASA for 15 days.
先前已有报道称白细胞浸润于静脉曲张管壁。本研究旨在调查对照组及静脉曲张患者中促炎细胞因子和趋化因子的表达情况，并测试在切除静脉曲张之前用乙酰水杨酸（ASA）治疗静脉曲张患者对细胞因子表达的影响。
在手术过程中从两组患者身上获取静脉切片，并进行核糖核酸酶保护分析（RPA）以评估趋化因子的表达。第一组包括因创伤接受截肢手术的健康患者的非曲张大隐静脉。从接受手术治疗的原发性静脉曲张患者身上获取曲张静脉，这些患者未接受药物治疗（第二组）或在手术前15天接受300毫克/天（-1）的ASA治疗（第三组）。
非曲张静脉组成性地表达低水平的单核细胞趋化蛋白（MCP-1）和白细胞介素（IL）-8信使核糖核酸。曲张静脉具有独特的趋化因子表达模式，因为检测到MCP-1和IL-8的显著上调以及IP-10、RANTES、MIP-1α和MIP-1β信使核糖核酸的明显表达。去除内皮细胞并未改变这种模式。从接受ASA治疗的患者身上获取的曲张静脉显示趋化因子表达持续下降，尽管未达到统计学意义。
与对照静脉相比，曲张静脉中几种趋化因子的表达增加。用ASA治疗15天后观察到活化有非显著性降低。
