Wahyuni Agnesia Endang Tri Hastuti, Ramandani Dhasia, Prakasita Vinsa Cantya, Widyarini Sitarina
Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Department of Biotechnology and Veterinary, Vocational College, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Vet World. 2019 Jul;12(7):972-977. doi: 10.14202/vetworld.2019.972-977. Epub 2019 Jul 5.
Infectious coryza is caused by . In Indonesia, this infection results in a 10%-40% decrease in egg production by laying hens. This study was conducted to determine the effectiveness of tetravalent coryza vaccine contained bacterin serovars A, B, C2, and C3; strain A-221, B-Spross, C2-Modesto, and C-3-Akko in layers based on antibody titer and clinical signs using a post-challenge test.
Forty four-week-old Lohmanns strain chickens were used in this study. Forty chickens were divided into four groups for serological and challenge test: Group 1 (unvaccinated and challenged by serovar A), Group 2 (unvaccinated and challenged by serovar B), Group 3 (vaccinated and challenged by serovar A), and Group 4 (vaccinated and challenged by serovar B). Vaccination was done using the tetravalent vaccine in oil-emulsion adjuvant contained bacterin serovars A, B, C2, and C3; strain A-221, B-Spross, C2-Modesto, and C-3-Akko. Vaccination was performed at day 1 and booster was done at day 14. Blood serum was collected on days 0, 14, and 28 for the hemagglutination-hemagglutination inhibition (HI) test. The challenge test was given at day 29 through intranasal administration using serovars A-L2447 and B-L1710 approximately 6×10 CFU/mL. Clinical signs were observed for 14 days post-infection. At the end of the study, chickens were euthanized, and pathological features of the infraorbital sinus, facial skin, and trachea were recorded.
Data analysis of antibody titers and pathological changes was performed descriptively, while clinical symptom scores were analyzed non-parametrically with the Mann-Whitney U-test using SPSS version 21. At days 14 and 28 post-vaccination, the antibody titer in Group 3 was 5 HI and 20 HI, respectively. However, the antibody titers in Group 4 at 28 days post-vaccination were 0 HI. Clinical observations, the vaccinated groups that were challenged with serovars A and B showed clinical symptoms on days 4 and 6 post-infection, namely mild unilateral facial edema and severe bilateral facial edema, respectively. Clinical signs in Groups 3 and 4 were less severe than in Groups 1 and 2 (p<0.05). Pathological examination findings supported clinical observations and serological testing.
Tetravalent coryza vaccine in chickens has efficacy to protect against the challenge test of serovars A and B isolated from Indonesia.
传染性鼻炎由……引起。在印度尼西亚，这种感染导致产蛋母鸡的产蛋量下降10%-40%。本研究旨在通过攻毒后试验，根据抗体效价和临床症状来确定含有血清型A、B、C2和C3；菌株A-221、B-Spross、C2-Modesto和C-3-Akko的四价鼻炎疫苗在蛋鸡中的有效性。
本研究使用了44周龄的罗曼鸡。40只鸡被分为四组进行血清学和攻毒试验：第1组（未接种疫苗，用血清型A攻毒），第2组（未接种疫苗，用血清型B攻毒），第3组（接种疫苗，用血清型A攻毒），第4组（接种疫苗，用血清型B攻毒）。使用含有血清型A、B、C2和C3；菌株A-221、B-Spross、C2-Modesto和C-3-Akko的油乳剂佐剂四价疫苗进行接种。在第1天进行接种，在第14天进行加强免疫。在第0、14和28天采集血清进行血凝-血凝抑制（HI）试验。在第29天通过鼻内接种约6×10 CFU/mL的血清型A-L2447和B-L1710进行攻毒试验。感染后观察14天的临床症状。在研究结束时，对鸡实施安乐死，并记录眶下窦、面部皮肤和气管的病理特征。
对抗体效价和病理变化进行描述性数据分析，而使用SPSS 21版通过Mann-Whitney U检验对临床症状评分进行非参数分析。在接种疫苗后的第14天和第28天，第3组的抗体效价分别为5 HI和20 HI。然而，第4组在接种疫苗后28天的抗体效价为0 HI。临床观察发现，用血清型A和B攻毒的接种组在感染后第4天和第6天出现临床症状，分别为轻度单侧面部水肿和严重双侧面部水肿。第3组和第4组的临床症状比第1组和第2组轻（p<0.05）。病理检查结果支持临床观察和血清学检测。
鸡用四价鼻炎疫苗对来自印度尼西亚的血清型A和B的攻毒试验具有保护作用。
