神经节阻断剂增强豚鼠神经介导的支气管收缩：感觉神经肽的可能作用。

Ganglion-blocking agents enhance neurally mediated bronchoconstriction in the guinea-pig: possible role of sensory neuropeptides.

作者信息

Del Monte M, Omini C, Subissi A

机构信息

Department of Pharmacology, Laboratori Guidotti S.p.A., Pisa, Italy.

出版信息

Eur J Pharmacol. 1990 May 16;180(2-3):239-45. doi: 10.1016/0014-2999(90)90307-r.

DOI:10.1016/0014-2999(90)90307-r

PMID:1973115

Abstract

The effect of ganglion blockade by hexamethonium bromide (0.1-100 mumol.kg-1) and pentolinium tartrate (0.01-3 mumol.kg-1) on the bronchoconstriction induced by vagal nerve stimulation (15 Hz, 0.2 ms, 3 s, 7-20 V) was evaluated in the anaesthetized guinea-pig. Both ganglion-blocking agents potentiated this response dose dependently. When the neural bronchoconstriction was suppressed by atropine, hexamethonium restored this response dose dependently. Hexamethonium produced inhibitory effects on vagally induced bronchoconstriction in capsaicin-desensitized and in propranolol- or reserpine-pretreated guinea-pigs. Propranolol (0.03-3 mumol.kg-1) produced a marked dose-dependent increase of neural bronchoconstriction (which was markedly reduced, about 10 times) in capsaicin-desensitized animals. Our results show that ganglion-blocking agents potentiate neural bronchoconstriction in the guinea-pig and that sensory neuropeptides may have a role in this effect. Moreover, beta-adrenergic modulation of the release of neuropeptides from vagal sensory fibers is suggested.

摘要

在麻醉的豚鼠中，评估了溴化六甲铵（0.1 - 100 μmol·kg⁻¹）和酒石酸喷托铵（0.01 - 3 μmol·kg⁻¹）对迷走神经刺激（15 Hz，0.2 ms，3 s，7 - 20 V）诱导的支气管收缩的神经节阻滞作用。两种神经节阻滞剂均剂量依赖性地增强了这种反应。当神经源性支气管收缩被阿托品抑制时，六甲铵剂量依赖性地恢复了这种反应。六甲铵对辣椒素脱敏的以及普萘洛尔或利血平预处理的豚鼠的迷走神经诱导的支气管收缩产生抑制作用。在辣椒素脱敏的动物中，普萘洛尔（0.03 - 3 μmol·kg⁻¹）使神经源性支气管收缩显著剂量依赖性增加（显著降低，约10倍）。我们的结果表明，神经节阻滞剂可增强豚鼠的神经源性支气管收缩，并且感觉神经肽可能在此效应中起作用。此外，提示了β - 肾上腺素能对迷走感觉纤维释放神经肽的调节作用。