Cox James, Williams Sarah, Grove Kevin, Lane Robert H, Aagaard-Tillery Kjersti M
Metabolomics Core Research Facility, University of Utah Health Sciences, Salt Lake City, UT, USA.
Am J Obstet Gynecol. 2009 Sep;201(3):281.e1-9. doi: 10.1016/j.ajog.2009.06.041.
To characterize the serum metabolome of a primate model of in utero high-fat exposure.
Serum from maternal and fetal (e130) macaque monkeys exposed to either a high-fat or control diet were analyzed by gas chromatography-mass spectrometry. Multivariate data analysis was performed to reduce the generated data set. Candidate metabolites were further analyzed for significance by using the analysis of variance and comparative t tests.
Approximately 1300 chromatographic features were detected. Through multivariate data analysis this number was reduced to 60 possible metabolites. With the use of comparative t tests, 22 metabolites had statistical significance (P < .05) over the entire study. By virtue of maternal high-fat diet alone, fetal phenotypic differences are accompanied by altered metabolite concentrations of 7 metabolites (P < .05).
In utero high-fat diet exposure is associated with an altered fetal epigenome and parlays a characteristic modification in the fetal metabolite profile.
描述子宫内高脂暴露灵长类动物模型的血清代谢组特征。
采用气相色谱 - 质谱分析法对暴露于高脂饮食或对照饮食的母猴和胎猴（妊娠130天）血清进行分析。进行多变量数据分析以减少生成的数据集。通过方差分析和比较t检验进一步分析候选代谢物的显著性。
检测到约1300个色谱特征。通过多变量数据分析，该数量减少到60种可能的代谢物。使用比较t检验，在整个研究中22种代谢物具有统计学意义（P <.05）。仅由于母体高脂饮食，胎儿表型差异伴随着7种代谢物的代谢物浓度改变（P <.05）。
子宫内高脂饮食暴露与胎儿表观基因组改变有关，并使胎儿代谢物谱发生特征性改变。
