Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, Japan.
Dev Comp Immunol. 2010 Feb;34(2):133-40. doi: 10.1016/j.dci.2009.08.013. Epub 2009 Sep 11.
Mycobacteriosis, caused by Mycobacterium sp., results in severe loss of fish production in Japan's aquaculture industry. In this study, the effects of two vaccine candidates, Bacillus Calmette and Guèrin (BCG, an attenuated strain of Mycobacterium bovis) and formalin-killed cells of Mycobacterium sp. were evaluated in Japanese flounder, Paralichthys olivaceus. In the immediate response and tuberculin response, BCG-vaccinated fish showed higher gene expression levels of inflammatory cytokines such as IL-1beta, IL-6, IFN-gamma and TNFalpha. Furthermore, BCG vaccination conferred protective efficacy against Mycobacterium sp. infection in Japanese flounder. Transcriptome analysis using a Japanese flounder cDNA microarray revealed that BCG vaccination induced not only adaptive immunity against Mycobacterium sp. antigen but also the expression of non-specific bactericidal proteins such as lysozyme. These data suggest that BCG confers immunity to Mycobacterium sp. infection and is a potent vaccine candidate for fish mycobacteriosis.
分枝杆菌病由分枝杆菌引起，导致日本水产养殖业严重减产。在这项研究中，评估了两种疫苗候选物，卡介苗（一种减毒的牛分枝杆菌株）和福尔马林杀死的分枝杆菌细胞，在日本牙鲆中的效果。在即刻反应和结核菌素反应中，卡介苗接种的鱼显示出更高水平的炎症细胞因子基因表达，如 IL-1beta、IL-6、IFN-gamma 和 TNFalpha。此外，卡介苗接种对日本牙鲆的分枝杆菌感染提供了保护效力。使用日本牙鲆 cDNA 微阵列的转录组分析表明，卡介苗接种不仅诱导了对分枝杆菌抗原的适应性免疫，还诱导了溶菌酶等非特异性杀菌蛋白的表达。这些数据表明，卡介苗赋予了对分枝杆菌感染的免疫力，是鱼类分枝杆菌病的有效疫苗候选物。
