Lang Thierry, Lecoeur Hervé, Prina Eric
Institut Pasteur, Département de Parasitologie et Mycologie, Unité d' Immunophysiologie et Parasitisme Intracellulaire, 75724 Paris cedex 15, France.
Trends Parasitol. 2009 Oct;25(10):464-73. doi: 10.1016/j.pt.2009.07.006. Epub 2009 Sep 4.
The interactions between the Leishmania parasite and its mammalian host cells are strongly regulated dynamic processes that take place at the molecular, cellular and organ level. Several different interaction models have been developed to take advantage of the development of imaging technologies. Here, we first review how conventional imaging techniques have been applied to fixed Leishmania-loaded tissue and cell samples. Then, we show how transgenic Leishmania expressing fluorescent or bioluminescent reporters allowed characterization of their tissue and cell host niches. Most notably, the use of whole-body imaging or intravital microscopy techniques has allowed accurate real-time monitoring of parasites in their environment. Finally, we discuss how innovative imaging technologies will allow further refinement of our understanding of the interplay between Leishmania and its hosts.
利什曼原虫寄生虫与其哺乳动物宿主细胞之间的相互作用是在分子、细胞和器官水平上发生的受到强烈调控的动态过程。已经开发了几种不同的相互作用模型以利用成像技术的发展。在这里，我们首先回顾传统成像技术如何应用于固定的载有利什曼原虫的组织和细胞样本。然后，我们展示了表达荧光或生物发光报告基因的转基因利什曼原虫如何能够对其组织和细胞宿主生态位进行表征。最值得注意的是，全身成像或活体显微镜技术的使用使得能够在其环境中对寄生虫进行准确的实时监测。最后，我们讨论创新成像技术将如何使我们对利什曼原虫与其宿主之间相互作用的理解得到进一步完善。
