Ghiasvand Ali Reza, Shirzadi Hassan, Ghasemi Hossein Ali, Taherpour Kamran, Hasanvand Shokoufeh, Khatibjoo Ali
Department of Animal Science, Faculty of Agriculture, Ilam University, Ilam, Iran.
Department of Animal Science, Faculty of Agriculture, Ilam University, Ilam, Iran.
Poult Sci. 2025 Aug 13;104(11):105684. doi: 10.1016/j.psj.2025.105684.
This research examines the impact of different dietary arginine (Arg) concentrations on growth performance, carcass traits, gut morphology, and gene expression in Japanese quails aged 1 to 35 days. A total of 600 Japanese quail chicks were randomly allocated to five dietary treatments consisting of 0.75%, 1.0%, 1.25%, 1.50%, and 1.75% digestible Arg, with six replicate cages for each treatment and 20 birds housed per cage. Dietary digestible Arg concentrations of 1.0% or above led to improvements in growth performance, with the groups receiving 1.25% and 1.50% demonstrating the most pronounced increases in body weight and average daily gain (ADG) by day 35. The group receiving 1.25% digestible Arg had the most advantageous feed conversion ratio (FCR) and showed an increase in average daily feed intake. According to the one-slope linear broken-line regression model, the estimated breakpoint for ADG was 1.19% digestible Arg, while the breakpoint for FCR was 1.30% digestible Arg. Quadratic regression analysis further estimated maximal ADG at 1.42% digestible Arg and minimal FCR at 1.47% digestible Arg during the 1-35 day period. Furthermore, the 1.25% digestible Arg diet markedly improved gut morphology, especially in terms of jejunal villus height and surface area, which suggests an enhanced capacity for nutrient absorption. The group receiving 1.25% digestible Arg demonstrated superior carcass yield, significantly exceeding that of the 0.75% and 1.75% groups. Gene expression analysis revealed that hepatic HSP90 expression peaked in the 1.00% Arg group, but the expression levels of hepatic target of rapamycin (TOR) and ribosomal protein S6 kinase alpha-1 (RPS6KA1) were considerably elevated in the 1.50% and 1.75% groups. The treatments with 1.25% and 1.50% digestible Arg demonstrated the highest levels of kidney HSP70 expression. In conclusion, dietary supplementation at a concentration of 1.25% and more digestible Arg improved growth performance, carcass yield, gut morphology, and expression of genes related to growth and stress in Japanese quail, with model-based estimates refining the digestible Arg requirement range between 1.19% and 1.47%.
本研究考察了不同日粮精氨酸（Arg）浓度对1至35日龄日本鹌鹑生长性能、胴体性状、肠道形态及基因表达的影响。总共600只日本鹌鹑雏鸡被随机分配到五种日粮处理组，日粮可消化Arg浓度分别为0.75%、1.0%、1.25%、1.50%和1.75%，每种处理设六个重复笼，每笼饲养20只鸟。日粮可消化Arg浓度在1.0%及以上时可提高生长性能，到35日龄时，日粮可消化Arg浓度为1.25%和1.50%的组体重和平均日增重（ADG）增加最为显著。日粮可消化Arg浓度为1.25%的组饲料转化率（FCR）最有利，且平均日采食量增加。根据单斜率线性折线回归模型，ADG的估计断点为1.19%可消化Arg，FCR的断点为1.30%可消化Arg。二次回归分析进一步估计，在1至35日龄期间，可消化Arg浓度为1.42%时ADG最高，可消化Arg浓度为1.47%时FCR最低。此外，日粮可消化Arg浓度为1.25%显著改善了肠道形态，尤其是空肠绒毛高度和表面积，这表明营养物质吸收能力增强。日粮可消化Arg浓度为1.25%的组胴体产率更高，显著超过日粮可消化Arg浓度为0.75%和1.75%的组。基因表达分析显示，肝脏热休克蛋白90（HSP90）表达在可消化Arg浓度为1.00%的组达到峰值，但肝脏雷帕霉素靶蛋白（TOR）和核糖体蛋白S6激酶α-1（RPS6KA1）的表达水平在可消化Arg浓度为1.50%和1.75%的组显著升高。日粮可消化Arg浓度为1.25%和1.50%的处理组肾脏HSP70表达水平最高。总之，日粮添加浓度为1.25%及以上的可消化Arg可改善日本鹌鹑的生长性能、胴体产率、肠道形态以及与生长和应激相关基因的表达，基于模型的估计细化了可消化Arg的需求范围在1.19%至1.47%之间。
