Andersen J K, Saunders N A
Institute of Hygiene and Microbiology, Royal Veterinary and Agricultural University, Fredericksberg, Denmark.
J Med Microbiol. 1990 Jul;32(3):179-87. doi: 10.1099/00222615-32-3-179.
Intra-species restriction fragment length polymorphisms (RFLPs) of Yersinia enterocolitica were detected in assays with a cloned DNA fragment from Legionella pneumophila that included the 16S and 23S rRNA genes. By use of this method it was possible to identify different RFLP types within biogroups/serogroups which were indistinguishable by other means. Thus the 37 biogroup IV/serogroup O3 strains isolated worldwide from pig carcasses, pork and human patients, were subdivided into five different RFLP-types. Typing based on RFLP analysis was sensitive and independent of phenotypic characters. The method will be of value for the identification and evaluation of possible reservoirs and routes of infection.
在使用包含嗜肺军团菌16S和23S rRNA基因的克隆DNA片段进行的检测中，发现了小肠结肠炎耶尔森菌的种内限制性片段长度多态性（RFLP）。通过使用这种方法，可以在生物群/血清群内鉴定出通过其他方法无法区分的不同RFLP类型。因此，从世界各地猪胴体、猪肉和人类患者中分离出的37株生物群IV/血清群O3菌株被细分为五种不同的RFLP类型。基于RFLP分析的分型敏感且独立于表型特征。该方法对于识别和评估可能的感染源和传播途径具有重要价值。
