Saken E, Roggenkamp A, Aleksic S, Heesemann J
Institute for Hygiene and Microbiology, University of Würzburg, Germany.
J Med Microbiol. 1994 Nov;41(5):329-38. doi: 10.1099/00222615-41-5-329.
Enteropathogenic Yersinia enterocolitica is an important cause of human and animal disease. Phenotypic and genotypic characteristics currently used to identify Y. enterocolitica are not necessarily sufficient to differentiate pathogenic from non-pathogenic strains or to analyse the epidemiology of yersiniae at a molecular level. To improve the characterisation of Yersinia isolates, NotI restriction fragment length polymorphisms (RFLPs) of chromosomal DNA of more than 100 clinical, animal and environmental isolates were analysed in pulsed-field gel electrophoresis. Highly conserved RFLP patterns with fragments ranging from 15 to 400 kb were detected within each of 10 Y. enterocolitica serogroups tested. Determination of RFLP types makes it possible to discriminate between isolates of different Y. enterocolitica serogroups and other Yersinia spp. Moreover, NotI restriction endonuclease analysis allows even subtyping of strains belonging to a unique serogroup-biotype. Identification of NotI fragments hybridising with inv- or ail-homologous sequences was used as an additional discriminating marker. The results indicate that NotI RFLP typing can provide a powerful new tool for the differentiation of clinical Y. enterocolitica isolates.
肠道致病性小肠结肠炎耶尔森菌是人和动物疾病的重要病因。目前用于鉴定小肠结肠炎耶尔森菌的表型和基因型特征不一定足以区分致病菌株和非致病菌株，也不足以在分子水平上分析耶尔森菌的流行病学。为了改进耶尔森菌分离株的特征描述，在脉冲场凝胶电泳中分析了100多个临床、动物和环境分离株的染色体DNA的NotI限制性片段长度多态性（RFLP）。在所测试的10个小肠结肠炎耶尔森菌血清群中，每个血清群都检测到了高度保守的RFLP模式，片段大小从15到400 kb不等。RFLP类型的确定使得区分不同小肠结肠炎耶尔森菌血清群的分离株和其他耶尔森菌属成为可能。此外，NotI限制性内切酶分析甚至可以对属于独特血清群-生物型的菌株进行亚型分析。与inv或ail同源序列杂交的NotI片段的鉴定被用作额外的鉴别标记。结果表明，NotI RFLP分型可为临床小肠结肠炎耶尔森菌分离株的鉴别提供一种强大的新工具。
