Kapperud G, Nesbakken T, Aleksic S, Mollaret H H
Department of Bacteriology, National Institute of Public Health, Oslo, Norway.
J Clin Microbiol. 1990 Jun;28(6):1125-31. doi: 10.1128/jcm.28.6.1125-1131.1990.
Restriction endonuclease analysis of chromosomal DNA (REAC) was used to study polymorphism in restriction fragment patterns among Yersinia enterocolitica isolates belonging to serogroups O3, O5,27, O8, O9, O13, and O21. Using the enzyme HaeIII and electrophoresis on thin (0.75-mm) vertical 5% polyacrylamide gels, we were able to distinguish at least 22 DNA fragment patterns among the 72 strains examined. The method showed the greatest discriminatory power with regard to serogroup O8, within which as many as 10 different DNA fragment patterns were detected among the 16 strains examined. Compared with O8, serogroups O3 and O9 were relatively homogeneous with regard to REAC patterns. The discriminatory power of the method was compared with H-antigen typing, biotyping, phage typing, antimicrobial susceptibility typing, and restriction enzyme analysis of the virulence plasmid (REAP), by means of Simpson's index of diversity. The results showed that REAC and REAP constitute an effective supplement or alternative to conventional phenotypic methods for tracing epidemiologically related isolates of Y. enterocolitica. Our finding that human and porcine isolates exhibited the same REAC, REAP, and H-antigen patterns provides additional support for the hypothesis that pigs play an important role in the epidemiology of human Y. enterocolitica infection.
采用染色体DNA限制性内切酶分析（REAC）研究了小肠结肠炎耶尔森菌O3、O5,27、O8、O9、O13和O21血清群分离株的限制性片段模式多态性。使用HaeIII酶并在0.75毫米厚的垂直5%聚丙烯酰胺凝胶上进行电泳，我们能够在检测的72株菌株中区分出至少22种DNA片段模式。该方法对O8血清群显示出最大的鉴别力，在所检测的16株菌株中检测到多达10种不同的DNA片段模式。与O8相比，O3和O9血清群在REAC模式方面相对较为单一。通过辛普森多样性指数将该方法的鉴别力与H抗原分型、生物分型、噬菌体分型、抗菌药物敏感性分型以及毒力质粒的限制性酶切分析（REAP）进行了比较。结果表明，REAC和REAP是追踪小肠结肠炎耶尔森菌流行病学相关分离株的传统表型方法的有效补充或替代方法。我们发现人和猪的分离株表现出相同的REAC、REAP和H抗原模式，这为猪在人类小肠结肠炎耶尔森菌感染流行病学中起重要作用这一假说提供了额外支持。
