Dossus Laure, Becker Susen, Achaintre David, Kaaks Rudolf, Rinaldi Sabina
Division of Cancer Epidemiology, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, Heidelberg 69120, Germany.
J Immunol Methods. 2009 Oct 31;350(1-2):125-32. doi: 10.1016/j.jim.2009.09.001. Epub 2009 Sep 10.
Multiplex-based immunoassays (MIA) allow the simultaneous measurement of different cytokines in small sample volumes, but their applicability in samples from "healthy" subjects, as those participating in large-scale epidemiological studies is not well known. We compared measurements of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), interleukin-1 receptor antagonist (IL-1Ra), C-reactive protein (CRP) and soluble CD40L (sCD40L) obtained by MIA with those obtained by enzyme-linked immunosorbent assays (ELISA) in serum and plasma samples from 36 "healthy" subjects. We observed good correlations between measurements obtained by MIA and ELISA for IL-1Ra, CRP and sCD40L (r>0.80) but poor correlations for IL-6, TNF-alpha and IL-1beta (r<0.40). When comparing MIA plasma and serum measurements, very high correlations were obtained for CRP (r=0.98) and fairly good correlations for IL-1Ra (r=0.60). In conclusion, multiplex-based assays can give an accurate estimate of the relative risk in large-scale epidemiological studies but only for cytokines that are present in relatively large concentrations (ng/mL).
基于多重检测的免疫分析方法（MIA）能够在小样本体积中同时检测不同的细胞因子，但其在参与大规模流行病学研究的“健康”受试者样本中的适用性尚不清楚。我们比较了通过MIA在36名“健康”受试者的血清和血浆样本中获得的白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-1受体拮抗剂（IL-1Ra）、C反应蛋白（CRP）和可溶性CD40配体（sCD40L）的检测结果与通过酶联免疫吸附测定（ELISA）获得的结果。我们观察到，对于IL-1Ra、CRP和sCD40L，MIA和ELISA的检测结果之间具有良好的相关性（r>0.80），而对于IL-6、TNF-α和IL-1β，相关性较差（r<0.40）。比较MIA血浆和血清检测结果时，CRP的相关性非常高（r=0.98），IL-1Ra的相关性相当好（r=0.60）。总之，基于多重检测的分析方法能够在大规模流行病学研究中对相对风险给出准确估计，但仅适用于相对高浓度（ng/mL）存在的细胞因子。
