Department of Orthodontics, Thailand Excellence Center for Tissue Engineering, Chiang Mai University, Thailand.
Eur J Orthod. 2010 Feb;32(1):60-5. doi: 10.1093/ejo/cjp056. Epub 2009 Sep 13.
The aim of this study was to monitor changes in chondroitin sulphate (CS; WF6 epitope) levels in peri-miniscrew implant crevicular fluid (PMICF) during orthodontic loading. Ten patients (seven males and three females; aged 22.0 +/- 3.4 years), who required orthodontic treatment with extraction of all four premolar teeth, participated in the study. Twenty miniscrew implants (used as orthodontic anchorage) were placed, two in each patient, buccally and bilaterally in the alveolar bone between the roots of the maxillary posterior teeth. Sentalloy closed-coil springs (50 g) were used to load the miniscrew implants and to move the maxillary canines distally. During the unloaded period, PMICF samples were collected on days 1, 3, 5, and 7 after miniscrew implant placement and on days 14, 21, 28, and 35 during the loaded period. Clinical mobility assessments of the miniscrew implants were recorded at each visit. The competitive enzyme-linked immunosorbent assay with monoclonal antibody WF6 was used to detect CS (WF6 epitope) levels in the PMICF samples. The differences between the CS (WF6 epitope) levels during the unloaded and loaded periods were determined by a Mann-Whitney U-test. During the loaded period, two miniscrew implants were considered to have failed. The CS (WF6 epitope) levels during the unloaded period ranged from 0.00 to 758.03 ng/ml and those during the loaded period from 0.00 to 1025.11 ng/ml. Medians of CS (WF6 epitope) levels, around 'immobile' miniscrew implants, between the unloaded and loaded periods were not significantly different (P = 0.07). CS (WF6 epitope) levels in PMICF can be detected and may be used as biomarkers for assessing alveolar bone remodelling around miniscrew implants during orthodontic loading.
本研究旨在监测正畸加载过程中peri-miniscrew 种植体周围液（PMICF）中硫酸软骨素（CS；WF6 表位）水平的变化。10 名患者（7 名男性和 3 名女性；年龄 22.0 +/- 3.4 岁）参与了这项研究，他们需要接受拔牙正畸治疗，拔除所有四颗前磨牙。在每个患者的上颌后牙根之间的牙槽骨中，双侧颊侧各放置 20 个微螺钉种植体（用作正畸锚固）。使用 Sentalloy 闭圈弹簧（50g）加载微螺钉种植体并将上颌尖牙远移。在未加载期间，在微螺钉植入物放置后的第 1、3、5 和 7 天以及加载期间的第 14、21、28 和 35 天收集 PMICF 样本。每次就诊时均记录微螺钉种植体的临床活动度评估。使用单克隆抗体 WF6 的竞争性酶联免疫吸附试验检测 PMICF 样本中的 CS（WF6 表位）水平。通过 Mann-Whitney U 检验确定未加载和加载期间 CS（WF6 表位）水平之间的差异。在加载期间，有两个微螺钉植入物被认为已经失效。未加载期 CS（WF6 表位）水平范围为 0.00 至 758.03ng/ml，加载期 CS（WF6 表位）水平范围为 0.00 至 1025.11ng/ml。未加载和加载期间围绕“不动”微螺钉植入物的 CS（WF6 表位）水平中位数无显着差异（P=0.07）。PMICF 中的 CS（WF6 表位）水平可以被检测到，并可用作评估正畸加载过程中微螺钉植入物周围牙槽骨重塑的生物标志物。
