Machawal Jitender, Kharbanda Om P, Duggal Ritu, Chauhan Shyam S, Samrit Vilas D
Orthodontics, All India Institute of Medical Sciences, New Delhi, IND.
Orthodontics, Ramaiah University of Applied Sciences, Karnataka, IND.
Cureus. 2023 Mar 13;15(3):e36060. doi: 10.7759/cureus.36060. eCollection 2023 Mar.
To assess the levels of Pentraxin-3 (PTX3) in peri-miniscrew implant crevicular fluid (PMICF) before and after orthodontic force application Material and Methods: This study included 40 miniscrew implants (MSI) sites in 11 orthodontic patients with high arch discrepancy requiring first premolar extraction using maximum anchorage mechanics for the retraction of anterior teeth. After alignment, the en-masse anterior retraction was carried out using the MSI-supported direct anchorage method. PMICF was collected from the crevice of MSI using Periopaper strips 1.2µl ( Inc. USA) after one hour, 24 hours, and three weeks of MSI insertion and after one hour, 24 hours, seven days, three weeks, and six weeks of the force application. Samples were quantitatively analyzed for PTX3 levels through enzyme-linked immunosorbent assay (ELISA).
The trend in the change of PTX3 levels was evaluated using the Wilcoxon signed-rank test. The mean concentration of PTX3 immediately after MSI insertion was 1.19 ng/ml, significantly higher than after 3 weeks after MSI insertion (0.72 ng/ml), which may correspond to the baseline. After loading, the mean PTX3 concentration increased significantly with the peak at 24 hrs (1.28 ng/ml), followed by a gradual decline till the completion of the study (0.5 ng/ml).
After MSI insertion, a rise in PTX3 levels in PMICF suggests an underlying inflammatory process. The slow decline in PTX3 level and return to the baseline after loading suggests an adaptive bone response to the stimulus.
评估正畸力施加前后微型螺钉种植体周围龈沟液（PMICF）中五聚素3（PTX3）的水平。
本研究纳入了11例正畸患者的40个微型螺钉种植体（MSI）部位，这些患者牙弓差异较大，需要拔除第一前磨牙，采用最大支抗力学方法内收前牙。排齐后，使用MSI支持的直接支抗方法进行整体前牙内收。在MSI植入后1小时、24小时和3周，以及施加力后1小时、24小时、7天、3周和6周，使用美国Periopaper公司生产的1.2µl试纸条从MSI的龈沟中收集PMICF。通过酶联免疫吸附测定（ELISA）对样本中的PTX3水平进行定量分析。
采用Wilcoxon符号秩检验评估PTX3水平的变化趋势。MSI植入后即刻PTX3的平均浓度为1.19 ng/ml，显著高于植入后3周（0.72 ng/ml），后者可能对应基线水平。加载力后，PTX3的平均浓度显著升高，在24小时达到峰值（1.28 ng/ml），随后逐渐下降直至研究结束（0.5 ng/ml）。
MSI植入后，PMICF中PTX3水平升高提示存在潜在的炎症过程。加载力后PTX3水平缓慢下降并恢复至基线水平，提示骨组织对刺激的适应性反应。
