Langness U, Nissen R
Res Exp Med (Berl). 1977 Jul 18;170(3):295-302. doi: 10.1007/BF01851440.
During the different stages of granuloma formation peptidyl prolyl hydroxylase activity and neutral salt soluble collagen were determined in the granuloma tissue. In parallel peptidyl prolyl hydroxylase activity, "collagen-like protein", and free hydroxyproline were measured in the serum of the same animals at correspondant time distances. A close correlation was found between the enzyme activity and the other parameters of collagen synthesis. These results confirm the conclusion that hydroxylation of polypeptide may be a rate limiting step in collagen biosynthesis. In addition the investigations revealed a close correlation between enzyme activity in the tissue and enzyme activity in the serum.
在肉芽肿形成的不同阶段，测定肉芽肿组织中的肽基脯氨酰羟化酶活性和中性盐溶性胶原。同时，在相同动物的血清中，于相应的时间间隔测量肽基脯氨酰羟化酶活性、“类胶原蛋白”和游离羟脯氨酸。发现酶活性与胶原合成的其他参数之间存在密切相关性。这些结果证实了多肽羟基化可能是胶原生物合成中的限速步骤这一结论。此外，研究还揭示了组织中的酶活性与血清中的酶活性之间存在密切相关性。
