Langness U, Hahn D, Walther T
Res Exp Med (Berl). 1978 Aug 15;173(2):177-85. doi: 10.1007/BF01851875.
Rats of Wistar strain, of same age, were kept on iron free diet up to nearly absolute iron deficiency in iron depots. Afterwards granuloma was produced by implantation of cotton pellets subcutaneously. For characterization of the collagen biosynthesis in the granuloma the neutral salt soluble collagen and the activity of the prolyl hydroxylase were measured in the granuloma 4, 8 and 12 days after cotton pellet implantation. At the same time "collagen-like" protein was determined in the serum of the animals. The enzyme activity was statistically significant lower in the granuloma of animals on iron deficiency than in the granuloma of the comparable groups on normal diet. Statistically significant higher concentration of neutral salt soluble collagen was found 8 and 12 days after cotton pellet implantation in the group on iron free diet. There was no significant difference as to the serum levels of "collagen-like" protein in serum.
选用同龄的Wistar品系大鼠，使其食用无铁饮食，直至铁储存几乎完全缺乏。之后，通过皮下植入棉球产生肉芽肿。为了表征肉芽肿中胶原蛋白的生物合成，在植入棉球后4、8和12天，测量肉芽肿中中性盐溶性胶原蛋白和脯氨酰羟化酶的活性。同时，测定动物血清中的“类胶原”蛋白。缺铁动物肉芽肿中的酶活性在统计学上显著低于正常饮食对照组肉芽肿中的酶活性。在无铁饮食组中，植入棉球后8天和12天，中性盐溶性胶原蛋白浓度在统计学上显著更高。血清中“类胶原”蛋白的水平没有显著差异。
