Department of Dermatology, Roskilde Hospital, Kogevej 7-13, Roskilde, Denmark.
Exp Dermatol. 2009 Dec;18(12):1001-9. doi: 10.1111/j.1600-0625.2009.00947.x.
Stereology is a set of mathematical and statistical tools to estimate three-dimensional (3-D) characteristics of objects from regular two-dimensional (2-D) sections. In medicine and biology, it can be used to estimate features such as cell volume, cell membrane surface area, total length of blood vessels per volume tissue and total number of cells. The unbiased quantification of these 3-D features allows for a better understanding of morphology in vivo compared with 2-D methods. This review provides an introduction to the field of stereology with specific emphasis on the application of stereology to dermatological research by supplying a short insight into the theoretical basis behind the technique and presenting previous dermatological studies in which stereology was an integral part. Both the theory supporting stereology and a practical approach in a dermatological setting are reviewed with the aim to provide the reader with the capability to better assess papers employing stereological estimators and to design stereological studies independently.
体视学是一组数学和统计学工具，用于从规则的二维（2-D）切片估计三维（3-D）物体的特征。在医学和生物学中，它可用于估计细胞体积、细胞膜表面积、单位组织体积内血管总长度和细胞总数等特征。与 2-D 方法相比，对这些 3-D 特征的无偏量化可更好地了解体内形态。本文综述了体视学领域，特别强调了体视学在皮肤科研究中的应用，简要介绍了该技术的理论基础，并介绍了体视学作为一个组成部分的先前皮肤科研究。本文综述了支持体视学的理论和皮肤科的实际应用方法，旨在使读者能够更好地评估使用体视学估计量的论文，并独立设计体视学研究。
