Hershfield M S, Snyder F F, Seegmiller J E
Science. 1977 Sep 23;197(4310):1284-7. doi: 10.1126/science.197600.
Mutants deficient in adenosine kinase or adenine phosphoribosyltransferase activities were selected from the WI-L2 line of human lymphoblasts. The adenosine kinase-deficient mutant was still as sensitive as its parent to growth inhibition caused by adenosine deaminase was inhibited. Similarly, the adenine phosphoribosyltransferase mutant remained sensitive to growth inhibition caused by adenine. Thus, the toxicity of adenine and adenosine to human lymphoblasts is not mediated by nucleotides to which they may be converted.
从人淋巴母细胞WI-L2系中筛选出腺苷激酶或腺嘌呤磷酸核糖转移酶活性缺陷的突变体。腺苷激酶缺陷型突变体对腺苷脱氨酶抑制所引起的生长抑制仍与其亲本一样敏感。同样，腺嘌呤磷酸核糖转移酶突变体对腺嘌呤所引起的生长抑制仍敏感。因此，腺嘌呤和腺苷对人淋巴母细胞的毒性不是由它们可能转化而成的核苷酸介导的。
