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黄曲霉群线粒体DNA的限制性内切酶分析:黄曲霉、寄生曲霉和诺米曲霉。

Restriction enzyme analysis of mitochondrial DNA of the Aspergillus flavus group: A. flavus, A. parasiticus, and A. nomius.

作者信息

Moody S F, Tyler B M

机构信息

Research School of Biological Sciences, Australian National University, Canberra, Australian Capital Territory.

出版信息

Appl Environ Microbiol. 1990 Aug;56(8):2441-52. doi: 10.1128/aem.56.8.2441-2452.1990.

DOI:10.1128/aem.56.8.2441-2452.1990
PMID:1976299
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC184747/
Abstract

Mitochondrial DNA restriction fragment length polymorphisms were identified that clearly distinguish Aspergillus flavus, A. parasiticus, and A. nomius. Mitochondrial DNAs of A. flavus and A. parasiticus were found to be circular, and their size was estimated size to be 32 kilobases. A restriction map was constructed for the mitochondrial genome of an A. parasiticus isolate by using four restriction endonucleases. Four genes tested were found to have the same order as in the mitochondrial genome of A. nidulans. The mitochondrial genome of A. nomius was estimated to be 33 kilobases.

摘要

已鉴定出线粒体DNA限制性片段长度多态性,可明确区分黄曲霉、寄生曲霉和诺米曲霉。发现黄曲霉和寄生曲霉的线粒体DNA呈环状,其大小估计为32千碱基。通过使用四种限制性内切酶构建了寄生曲霉分离株线粒体基因组的限制性图谱。测试的四个基因的顺序与构巢曲霉线粒体基因组中的顺序相同。诺米曲霉的线粒体基因组估计为33千碱基。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3e/184747/ba88abcd5efe/aem00089-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3e/184747/336bd07341d2/aem00089-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3e/184747/d6195c4cb7bd/aem00089-0177-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3e/184747/0dc6b28c28fa/aem00089-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3e/184747/ba88abcd5efe/aem00089-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3e/184747/336bd07341d2/aem00089-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3e/184747/d6195c4cb7bd/aem00089-0177-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3e/184747/0dc6b28c28fa/aem00089-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d3e/184747/ba88abcd5efe/aem00089-0182-a.jpg

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本文引用的文献

1
THE DETERMINATION OF AFLATOXINS IN COTTONSEED PRODUCTS.棉籽产品中黄曲霉毒素的测定
J Am Oil Chem Soc. 1965 Jun;42:471-5. doi: 10.1007/BF02540087.
2
Assembly of the mitochondrial membrane system: sequence analysis of a yeast mitochondrial ATPase gene containing the oli-2 and oli-4 loci.线粒体膜系统的组装:包含oli - 2和oli - 4位点的酵母线粒体ATP酶基因的序列分析
Cell. 1980 Jun;20(2):507-17. doi: 10.1016/0092-8674(80)90637-6.
3
An improved medium for the detection of Aspergillus flavus and A. parasiticus.一种用于检测黄曲霉和寄生曲霉的改良培养基。
利用单限制酶MwoI开发用于鉴定医学上重要曲霉菌种的RFLP-PCR方法。
Braz J Microbiol. 2014 Aug 29;45(2):503-7. doi: 10.1590/s1517-83822014000200018. eCollection 2014.
4
Simple and highly discriminatory VNTR-based multiplex PCR for tracing sources of Aspergillus flavus isolates.用于追踪黄曲霉分离株来源的简单且具有高度区分能力的基于 VNTR 的多重 PCR 方法。
PLoS One. 2012;7(9):e44204. doi: 10.1371/journal.pone.0044204. Epub 2012 Sep 17.
5
An insight into the distribution, genetic diversity, and mycotoxin production of Aspergillus section Flavi in soils of pistachio orchards.深入了解在开心果果园土壤中黄曲霉属(Aspergillus section Flavi)的分布、遗传多样性和产毒情况。
Folia Microbiol (Praha). 2012 Jan;57(1):27-36. doi: 10.1007/s12223-011-0090-5. Epub 2011 Dec 14.
6
A review molecular typing methods for Aspergillus flavus isolates.曲霉属黄曲霉分离株的分子分型方法研究进展。
Mycopathologia. 2011 Aug;172(2):83-93. doi: 10.1007/s11046-011-9406-x. Epub 2011 Mar 3.
7
High resolution genotyping of clinical Aspergillus flavus isolates from India using microsatellites.利用微卫星对来自印度的临床烟曲霉分离株进行高分辨率基因分型。
PLoS One. 2011 Jan 17;6(1):e16086. doi: 10.1371/journal.pone.0016086.
8
Mycotic keratitis due to Aspergillus nomius.由nomius 曲霉引起的真菌性角膜炎。
J Clin Microbiol. 2009 Oct;47(10):3382-5. doi: 10.1128/JCM.01051-09. Epub 2009 Aug 26.
9
Heteroduplex panel analysis, a novel method for genetic identification of Aspergillus Section Flavi strains.异源双链体分析,一种用于鉴定黄曲霉群菌株的新型基因鉴定方法。
Appl Environ Microbiol. 2001 Sep;67(9):4084-90. doi: 10.1128/AEM.67.9.4084-4090.2001.
10
Use of a repetitive DNA probe to type clinical and environmental isolates of Aspergillus flavus from a cluster of cutaneous infections in a neonatal intensive care unit.使用重复DNA探针鉴定新生儿重症监护病房中一系列皮肤感染病例的临床和环境黄曲霉菌株。
J Clin Microbiol. 2000 Oct;38(10):3612-8. doi: 10.1128/JCM.38.10.3612-3618.2000.
J Appl Bacteriol. 1983 Feb;54(1):109-14. doi: 10.1111/j.1365-2672.1983.tb01307.x.
4
Isolation of DNA from filamentous fungi and separation into nuclear, mitochondrial, ribosomal, and plasmid components.从丝状真菌中分离DNA并将其分离为核、线粒体、核糖体和质粒成分。
Anal Biochem. 1983 Dec;135(2):416-22. doi: 10.1016/0003-2697(83)90704-2.
5
Order and orientation of genic sequences in circular mitochondrial DNA from Saccharomyces exiguus: implications for evolution of yeast mtDNAs.嗜杀啤酒酵母环状线粒体DNA中基因序列的顺序与方向:对酵母线粒体DNA进化的启示
J Mol Evol. 1983;19(5):333-41. doi: 10.1007/BF02101636.
6
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
7
Maximum likelihood estimation of the number of nucleotide substitutions from restriction sites data.基于限制性酶切位点数据的核苷酸替换数的最大似然估计。
Genetics. 1983 Sep;105(1):207-17. doi: 10.1093/genetics/105.1.207.
8
Structural variations and optional introns in the mitochondrial DNAs of Neurospora strains isolated from nature.从自然界分离的脉孢菌菌株线粒体DNA中的结构变异和可变内含子。
Plasmid. 1983 Jan;9(1):53-70. doi: 10.1016/0147-619x(83)90031-8.
9
Complete DNA sequence coding for the large ribosomal RNA of yeast mitochondria.编码酵母线粒体大核糖体RNA的完整DNA序列。
Nucleic Acids Res. 1983 Jan 25;11(2):339-48. doi: 10.1093/nar/11.2.339.
10
Restriction enzyme analysis of mitochondrial DNA of members of the genus Aspergillus as an aid in taxonomy.曲霉属成员线粒体DNA的限制性酶切分析在分类学中的应用
J Gen Microbiol. 1982 Mar;128(3):471-6. doi: 10.1099/00221287-128-3-471.